{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yawata M"],"funding":["NIAID NIH HHS"],"pagination":["2369-80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2532809"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112(6)"],"pubmed_abstract":["Variegated expression of 6 inhibitory HLA class I-specific receptors on primary NK cells was studied using high-dimension flow cytometry in 58 humans to understand the structure and function of NK-cell repertoires. Sixty-four subsets expressing all possible receptor com-binations were present in each repertoire, and the frequency of receptor-null cells varied among the donors. Enhancement in missing-self response between NK subsets varied substantially where subset responses were defined by donor KIR/HLA allotypes, reflecting the differences in interaction between inhibitory receptors and their ligands. This contrasted to the enhancement conferred by NKG2A, which was constant and of intermediate strength. We infer a mechanism that modulates frequencies of the NK subsets displaying diverse levels of missing-self response, a system that reduces the presence of KIR-expressing subsets that display either too strong or too weak a response and effectively replaces them with NKG2A-expressing cells in the repertoire. Through this high-resolution analysis of inhibitory receptor expression, 5 types of NK-cell repertoire were defined by their content of NKG2A(+)/NKG2A(-) cells, frequency of receptor-null cells, and degree of KIR receptor coexpression. The analyses provide new perspective on how personalized human NK-cell repertoires are structured."],"journal":["Blood"],"pubmed_title":["MHC class I-specific inhibitory receptors and their ligands structure diverse human NK-cell repertoires toward a balance of missing self-response."],"pmcid":["PMC2532809"],"funding_grant_id":["R01 AI022039","AI-31168","AI-22039","R01 AI031168","R01 AI017892","AI-17892"],"pubmed_authors":["Partheniou F","Little AM","Parham P","Draghi M","Yawata M","Yawata N"],"additional_accession":[]},"is_claimable":false,"name":"MHC class I-specific inhibitory receptors and their ligands structure diverse human NK-cell repertoires toward a balance of missing self-response.","description":"Variegated expression of 6 inhibitory HLA class I-specific receptors on primary NK cells was studied using high-dimension flow cytometry in 58 humans to understand the structure and function of NK-cell repertoires. Sixty-four subsets expressing all possible receptor com-binations were present in each repertoire, and the frequency of receptor-null cells varied among the donors. Enhancement in missing-self response between NK subsets varied substantially where subset responses were defined by donor KIR/HLA allotypes, reflecting the differences in interaction between inhibitory receptors and their ligands. This contrasted to the enhancement conferred by NKG2A, which was constant and of intermediate strength. We infer a mechanism that modulates frequencies of the NK subsets displaying diverse levels of missing-self response, a system that reduces the presence of KIR-expressing subsets that display either too strong or too weak a response and effectively replaces them with NKG2A-expressing cells in the repertoire. Through this high-resolution analysis of inhibitory receptor expression, 5 types of NK-cell repertoire were defined by their content of NKG2A(+)/NKG2A(-) cells, frequency of receptor-null cells, and degree of KIR receptor coexpression. The analyses provide new perspective on how personalized human NK-cell repertoires are structured.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Sep","modification":"2026-03-16T16:22:16.61Z","creation":"2025-08-30T03:10:15.018Z"},"accession":"S-EPMC2532809","cross_references":{"pubmed":["18583565"],"doi":["10.1182/blood-2008-03-143727"]}}