biostudies-literatureUnknown28(19)Venables JPAlternative splicing is a key mechanism regulating gene expression, and it is often used to produce antagonistic activities particularly in apoptotic genes. Heterogeneous nuclear ribonucleoparticle (hnRNP) proteins form a family of RNA-binding proteins that coat nascent pre-mRNAs. Many but not all major hnRNP proteins have been shown to participate in splicing control. The range and specificity of hnRNP protein action remain poorly documented, even for those affecting splice site selection. We used RNA interference and a reverse transcription-PCR screening platform to examine the implications of 14 of the major hnRNP proteins in the splicing of 56 alternative splicing events in apoptotic genes. Out of this total of 784 alternative splicing reactions tested in three human cell lines, 31 resMolecular and cellular biology6033-43https://www.ebi.ac.uk/biostudies/studies/S-EPMC2547008biostudies-literatureMultiple and specific mRNA processing targets for the major human hnRNP proteins.PMC2547008Klinck RPrinos PPaquet ERKoh CSLapointe EBramard ACouture SWatier VElela SALucier JFFroehlich UVenables JPInkel LDurand MTremblay KGervais-Bird JChabot BfalseMultiple and specific mRNA processing targets for the major human hnRNP proteins.Alternative splicing is a key mechanism regulating gene expression, and it is often used to produce antagonistic activities particularly in apoptotic genes. Heterogeneous nuclear ribonucleoparticle (hnRNP) proteins form a family of RNA-binding proteins that coat nascent pre-mRNAs. Many but not all major hnRNP proteins have been shown to participate in splicing control. The range and specificity of hnRNP protein action remain poorly documented, even for those affecting splice site selection. We used RNA interference and a reverse transcription-PCR screening platform to examine the implications of 14 of the major hnRNP proteins in the splicing of 56 alternative splicing events in apoptotic genes. Out of this total of 784 alternative splicing reactions tested in three human cell lines, 31 res2008-01-01T00:00:00Z2008 Oct2025-04-18T16:28:15.253Z2019-03-27T00:16:42ZS-EPMC25470081864486410.1128/mcb.00726-0810.1128/MCB.00726-08