biostudies-literatureUnknown19(10)Krick RAutophagy is a diverse family of processes that transport cytoplasm and organelles into the lysosome/vacuole lumen for degradation. During macroautophagy cargo is packaged in autophagosomes that fuse with the lysosome/vacuole. During microautophagy cargo is directly engulfed by the lysosome/vacuole membrane. Piecemeal microautophagy of the nucleus (PMN) occurs in Saccharomyces cerevisiae at nucleus-vacuole (NV) junctions and results in the pinching-off and release into the vacuole of nonessential portions of the nucleus. Previous studies concluded macroautophagy ATG genes are not absolutely required for PMN. Here we report using two biochemical assays that PMN is efficiently inhibited in atg mutant cells: PMN blebs are produced, but vesicles are rarely released into the vacuole lumen. ElecMolecular biology of the cell4492-505https://www.ebi.ac.uk/biostudies/studies/S-EPMC2555948biostudies-literaturePiecemeal microautophagy of the nucleus requires the core macroautophagy genes.PMC2555948Eskelinen ELPrick TMuehe YBremer SThumm MMillen JGoldfarb DSKrick RSchlotterhose PfalsePiecemeal microautophagy of the nucleus requires the core macroautophagy genes.Autophagy is a diverse family of processes that transport cytoplasm and organelles into the lysosome/vacuole lumen for degradation. During macroautophagy cargo is packaged in autophagosomes that fuse with the lysosome/vacuole. During microautophagy cargo is directly engulfed by the lysosome/vacuole membrane. Piecemeal microautophagy of the nucleus (PMN) occurs in Saccharomyces cerevisiae at nucleus-vacuole (NV) junctions and results in the pinching-off and release into the vacuole of nonessential portions of the nucleus. Previous studies concluded macroautophagy ATG genes are not absolutely required for PMN. Here we report using two biochemical assays that PMN is efficiently inhibited in atg mutant cells: PMN blebs are produced, but vesicles are rarely released into the vacuole lumen. Elec2008-01-01T00:00:00Z2008 Oct2025-04-05T13:17:24.767Z2019-03-27T00:18:38ZS-EPMC25559481870170410.1091/mbc.e08-04-0363