{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["52(10)"],"submitter":["Petrella S"],"pubmed_abstract":["Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum cephalosporins and low susceptibility to imipenem were investigated. Both strains harbored the plasmid-contained bla(TEM-1) and bla(KPC-2) genes. bla(KLUC-2), encoding a mutant of the chromosomal beta-lactamase of Kluyvera cryocrescens, was also identified at a plasmid location in E. cloacae 7506, suggesting the ISEcp1-assisted escape of bla(KLUC) from the chromosome. Determination of the KPC-2 structure at 1.6 A revealed that the binding site was occupied by the C-terminal (C-ter) residues coming from a symmetric KPC-2 monomer, with the ultimate C-ter Glu interacting with Ser130, Lys234, Thr235, and Thr237 in the active site. "],"journal":["Antimicrobial agents and chemotherapy"],"pagination":["3725-36"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2565876"],"repository":["biostudies-literature"],"pubmed_title":["Genetic and structural insights into the dissemination potential of the extremely broad-spectrum class A beta-lactamase KPC-2 identified in an Escherichia coli strain and an Enterobacter cloacae strain isolated from the same patient in France."],"pmcid":["PMC2565876"],"pubmed_authors":["Ziental-Gelus N","Renard M","Sougakoff W","Jarlier V","Mayer C","Petrella S"],"additional_accession":[]},"is_claimable":false,"name":"Genetic and structural insights into the dissemination potential of the extremely broad-spectrum class A beta-lactamase KPC-2 identified in an Escherichia coli strain and an Enterobacter cloacae strain isolated from the same patient in France.","description":"Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum cephalosporins and low susceptibility to imipenem were investigated. Both strains harbored the plasmid-contained bla(TEM-1) and bla(KPC-2) genes. bla(KLUC-2), encoding a mutant of the chromosomal beta-lactamase of Kluyvera cryocrescens, was also identified at a plasmid location in E. cloacae 7506, suggesting the ISEcp1-assisted escape of bla(KLUC) from the chromosome. Determination of the KPC-2 structure at 1.6 A revealed that the binding site was occupied by the C-terminal (C-ter) residues coming from a symmetric KPC-2 monomer, with the ultimate C-ter Glu interacting with Ser130, Lys234, Thr235, and Thr237 in the active site. ","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Oct","modification":"2025-04-05T11:35:50.99Z","creation":"2019-03-27T00:18:50Z"},"accession":"S-EPMC2565876","cross_references":{"pubmed":["18625772"],"doi":["10.1128/aac.00163-08","10.1128/AAC.00163-08"]}}