biostudies-literatureFoy RLNIDDK NIH HHSNIEHS NIH HHSNCI NIH HHSCIHR28817-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC2570895biostudies-literatureUnknown283(43)Regulation of global chromatin acetylation is important for chromatin remodeling. A small family of Jade proteins includes Jade-1L, Jade-2, and Jade-3, each bearing two mid-molecule tandem plant homology domain (PHD) zinc fingers. We previously demonstrated that the short isoform of Jade-1L protein, Jade-1, is associated with endogenous histone acetyltransferase (HAT) activity. It has been found that Jade-1L/2/3 proteins co-purify with a novel HAT complex, consisting of HBO1, ING4/5, and Eaf6. We investigated a role for Jade-1/1L in the HBO1 complex. When overexpressed individually, neither Jade-1/1L nor HBO1 affected histone acetylation. However, co-expression of Jade-1/1L and HBO1 increased acetylation of the bulk of endogenous histone H4 in epithelial cells in a synergistic manner, suggThe Journal of biological chemistryRole of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.PMC2570895R01 DK087910K08 DK08094687253-1R01 DK67569R01 DK067569R01 CA79830R01 ES1291764289-2R01 CA079830Saksouk NCayrou CCote JPanchenko MVSong IYVaziri CCohen HTFoy RLChitalia VCfalseRole of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.Regulation of global chromatin acetylation is important for chromatin remodeling. A small family of Jade proteins includes Jade-1L, Jade-2, and Jade-3, each bearing two mid-molecule tandem plant homology domain (PHD) zinc fingers. We previously demonstrated that the short isoform of Jade-1L protein, Jade-1, is associated with endogenous histone acetyltransferase (HAT) activity. It has been found that Jade-1L/2/3 proteins co-purify with a novel HAT complex, consisting of HBO1, ING4/5, and Eaf6. We investigated a role for Jade-1/1L in the HBO1 complex. When overexpressed individually, neither Jade-1/1L nor HBO1 affected histone acetylation. However, co-expression of Jade-1/1L and HBO1 increased acetylation of the bulk of endogenous histone H4 in epithelial cells in a synergistic manner, sugg2008-01-01T00:00:00Z2008 Oct2025-04-22T06:45:28.374Z2019-03-27T00:19:00ZS-EPMC25708951868471410.1074/jbc.M80140720010.1074/jbc.m801407200