biostudies-literatureUnknown18(16)Ilarraza-Lomeli RFunction of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate Dp71 role in myoneural synapses, we established a coculture model using PC12 cells and L6 myotubes and analyzed expression and localization of Dp71 and related proteins, utrophin and beta-dystroglycan, in PC12 cells. Confocal microscopy showed Dp71d isoform in PC12 nuclei, golgi-complex-like and endoplasmic reticulum-like structures, whereas Dp71ab concentrates at neurite tips and cytoplasm, colocalizing with beta-dystroglycan, utrophin, synaptophysin and acetylcholine receptors. Evidences suggest that Dp71ab isoform, unlike Dp71d, may take part in neurite-related processes. This is the first work on Dp and members of Dp-associated protein complex roles in a cell-line bNeuroreport1657-61https://www.ebi.ac.uk/biostudies/studies/S-EPMC2580073biostudies-literatureDp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.PMC2580073Mornet DCisneros-Vega BMontanez CIlarraza-Lomeli RCervantes-Gomez Mde LfalseDp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.Function of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate Dp71 role in myoneural synapses, we established a coculture model using PC12 cells and L6 myotubes and analyzed expression and localization of Dp71 and related proteins, utrophin and beta-dystroglycan, in PC12 cells. Confocal microscopy showed Dp71d isoform in PC12 nuclei, golgi-complex-like and endoplasmic reticulum-like structures, whereas Dp71ab concentrates at neurite tips and cytoplasm, colocalizing with beta-dystroglycan, utrophin, synaptophysin and acetylcholine receptors. Evidences suggest that Dp71ab isoform, unlike Dp71d, may take part in neurite-related processes. This is the first work on Dp and members of Dp-associated protein complex roles in a cell-line b2007-01-01T00:00:00Z2007 Oct2025-06-01T00:53:37.063Z2025-06-01T00:53:37.063ZS-EPMC25800731792186310.1097/wnr.0b013e3282f0e42d10.1097/WNR.0b013e3282f0e42d