biostudies-literatureUnknown4Claus SPTo characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ-free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well-defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co-metabolic products such as hippurate (urine) and 5-aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in GMolecular systems biology219https://www.ebi.ac.uk/biostudies/studies/S-EPMC2583082biostudies-literatureSystemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes.PMC2583082Rezzi SRoss ACloarec OKochhar SMartin FPHolmes EClaus SPTsang TMWang YNicholson JKSkordi EfalseSystemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes.To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ-free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well-defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co-metabolic products such as hippurate (urine) and 5-aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in G2008-01-01T00:00:00Z20082025-04-26T00:33:48.185Z2019-03-27T00:19:21ZS-EPMC25830821885481810.1038/msb.2008.56