{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Henry LG"],"funding":["NIDCR NIH HHS"],"pagination":["7985-93"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2593216"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["190(24)"],"pubmed_abstract":["The persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. DNA damage is a major consequence of oxidative stress. Unlike the case for other organisms, our previous report suggests a role for a non-base excision repair mechanism for the removal of 8-oxo-7,8-dihydroguanine (8-oxo-G) in P. gingivalis. Because the uvrB gene is known to be important in nucleotide excision repair, the role of this gene in the repair of oxidative stress-induced DNA damage was investigated. A 3.1-kb fragment containing the uvrB gene was PCR amplified from the chromosomal DNA of P. gingivalis W83. This gene was insertionally inactivated using the ermF-ermAM antibiotic cassette and used to create a uvrB-deficient mutant by a"],"journal":["Journal of bacteriology"],"pubmed_title":["DNA repair of 8-oxo-7,8-dihydroguanine lesions in Porphyromonas gingivalis."],"pmcid":["PMC2593216"],"funding_grant_id":["R01 DE019730","R01 DE013664","R01 DE013664-06","R01 DE022508"],"pubmed_authors":["Zhang K","Sandberg L","Henry LG","Fletcher HM"],"additional_accession":[]},"is_claimable":false,"name":"DNA repair of 8-oxo-7,8-dihydroguanine lesions in Porphyromonas gingivalis.","description":"The persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. DNA damage is a major consequence of oxidative stress. Unlike the case for other organisms, our previous report suggests a role for a non-base excision repair mechanism for the removal of 8-oxo-7,8-dihydroguanine (8-oxo-G) in P. gingivalis. Because the uvrB gene is known to be important in nucleotide excision repair, the role of this gene in the repair of oxidative stress-induced DNA damage was investigated. A 3.1-kb fragment containing the uvrB gene was PCR amplified from the chromosomal DNA of P. gingivalis W83. This gene was insertionally inactivated using the ermF-ermAM antibiotic cassette and used to create a uvrB-deficient mutant by a","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Dec","modification":"2025-04-04T13:38:59.807Z","creation":"2019-03-27T00:19:37Z"},"accession":"S-EPMC2593216","cross_references":{"pubmed":["18849425"],"doi":["10.1128/jb.00919-08","10.1128/JB.00919-08"]}}