biostudies-literatureHenry LGNIDCR NIH HHS7985-93https://www.ebi.ac.uk/biostudies/studies/S-EPMC2593216biostudies-literatureUnknown190(24)The persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. DNA damage is a major consequence of oxidative stress. Unlike the case for other organisms, our previous report suggests a role for a non-base excision repair mechanism for the removal of 8-oxo-7,8-dihydroguanine (8-oxo-G) in P. gingivalis. Because the uvrB gene is known to be important in nucleotide excision repair, the role of this gene in the repair of oxidative stress-induced DNA damage was investigated. A 3.1-kb fragment containing the uvrB gene was PCR amplified from the chromosomal DNA of P. gingivalis W83. This gene was insertionally inactivated using the ermF-ermAM antibiotic cassette and used to create a uvrB-deficient mutant by aJournal of bacteriologyDNA repair of 8-oxo-7,8-dihydroguanine lesions in Porphyromonas gingivalis.PMC2593216R01 DE019730R01 DE013664R01 DE013664-06R01 DE022508Zhang KSandberg LHenry LGFletcher HMfalseDNA repair of 8-oxo-7,8-dihydroguanine lesions in Porphyromonas gingivalis.The persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. DNA damage is a major consequence of oxidative stress. Unlike the case for other organisms, our previous report suggests a role for a non-base excision repair mechanism for the removal of 8-oxo-7,8-dihydroguanine (8-oxo-G) in P. gingivalis. Because the uvrB gene is known to be important in nucleotide excision repair, the role of this gene in the repair of oxidative stress-induced DNA damage was investigated. A 3.1-kb fragment containing the uvrB gene was PCR amplified from the chromosomal DNA of P. gingivalis W83. This gene was insertionally inactivated using the ermF-ermAM antibiotic cassette and used to create a uvrB-deficient mutant by a2008-01-01T00:00:00Z2008 Dec2025-04-04T13:38:59.807Z2019-03-27T00:19:37ZS-EPMC25932161884942510.1128/jb.00919-0810.1128/JB.00919-08