{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Majumdar S"],"funding":["NIGMS NIH HHS"],"pagination":["5937-40"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2597205"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(22)"],"pubmed_abstract":["Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays."],"journal":["Bioorganic & medicinal chemistry letters"],"pubmed_title":["Phage display of functional, full-length human and viral membrane proteins."],"pmcid":["PMC2597205"],"funding_grant_id":["GM-069337","T34 GM069337","R01 GM078528"],"pubmed_authors":["Majumdar S","Mendez AS","Hajduczki A","Weiss GA"],"additional_accession":[]},"is_claimable":false,"name":"Phage display of functional, full-length human and viral membrane proteins.","description":"Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Nov","modification":"2026-03-16T16:28:55.163Z","creation":"2025-08-31T03:05:28.184Z"},"accession":"S-EPMC2597205","cross_references":{"pubmed":["18667306"],"doi":["10.1016/j.bmcl.2008.07.051"]}}