biostudies-literatureKennedy ANIBIB NIH HHSNCRR NIH HHSNCI NIH HHS27-35https://www.ebi.ac.uk/biostudies/studies/S-EPMC2597700biostudies-literatureUnknown124(1)Tumor cells do not constitutively exhibit invasive activity, but rather, can be transiently induced to adhere and form lesions. We report here that the expression of seprase, a dominant EDTA-resistant gelatinase in malignant tumors, is dependent on tumor cell exposure to type I collagen gel (TICg). The induced seprase expression of ovarian tumor cells influences their collagen contraction and invasion capability. Importantly, tumor cells with reduced seprase expression, due to manipulation by RNA interference, showed a reduction of TICg contraction in the gel contractility assay, inhibition of tumor cell invasion through TICg as shown by a transwell migration assay and inhibition of peritoneal membrane tumor lesion in a mouse model. In addition, mAb C27, an antibody against beta1 integrin,International journal of cancerElevation of seprase expression and promotion of an invasive phenotype by collagenous matrices in ovarian tumor cells.PMC2597700M01 RR010710-070061R01 CA039077-20R41 CA103467-01R01 CA039077-21M01 RR010710R01 CA039077-22R01 CA039077-23R01CA0039077R01 EB002065R01 EB002065-18R01 EB002065-17M01 RR010710-11R01 EB002065-19R01 EB002065-21R01EB002065R01 EB002065-20R01 CA039077Chen WTKennedy ADong HChen DfalseElevation of seprase expression and promotion of an invasive phenotype by collagenous matrices in ovarian tumor cells.Tumor cells do not constitutively exhibit invasive activity, but rather, can be transiently induced to adhere and form lesions. We report here that the expression of seprase, a dominant EDTA-resistant gelatinase in malignant tumors, is dependent on tumor cell exposure to type I collagen gel (TICg). The induced seprase expression of ovarian tumor cells influences their collagen contraction and invasion capability. Importantly, tumor cells with reduced seprase expression, due to manipulation by RNA interference, showed a reduction of TICg contraction in the gel contractility assay, inhibition of tumor cell invasion through TICg as shown by a transwell migration assay and inhibition of peritoneal membrane tumor lesion in a mouse model. In addition, mAb C27, an antibody against beta1 integrin,2009-01-01T00:00:00Z2009 Jan2025-04-21T21:01:21.058Z2019-06-06T20:40:04ZS-EPMC25977001882301010.1002/ijc.23871