biostudies-literatureWagner MCNIDDK NIH HHS35579-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC2602882biostudies-literatureUnknown283(51)Glomerular injury is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. Renal ischemia or the loss of blood flow to the kidneys has been widely associated with tubular and endothelial injury but rarely has been shown to induce podocyte damage and disruption of the slit diaphragm. In this study, we have used an in vivo rat ischemic model to demonstrate that renal ischemia induces podocyte effacement with loss of slit diaphragm and proteinuria. Biochemical analysis of the ischemic glomerulus shows that ischemia induces rapid loss of interaction between slit diaphragm junctional proteins Neph1 and ZO-1. To further understand the effect of ischemia on molecular interactions between slit diaphragm proteins, a cell culture model was employed to study thThe Journal of biological chemistryIschemic injury to kidney induces glomerular podocyte effacement and dissociation of slit diaphragm proteins Neph1 and ZO-1.PMC2602882K08 DK081403K08 DK081403-01R01 DK069408P50 DK061594K01 DK072047-03Rhodes GVerma RGattone VHolzman LBPruthi VWang EGarg PArif EWagner MCWean SENihalani DSaleem MAMolitoris BAfalseIschemic injury to kidney induces glomerular podocyte effacement and dissociation of slit diaphragm proteins Neph1 and ZO-1.Glomerular injury is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. Renal ischemia or the loss of blood flow to the kidneys has been widely associated with tubular and endothelial injury but rarely has been shown to induce podocyte damage and disruption of the slit diaphragm. In this study, we have used an in vivo rat ischemic model to demonstrate that renal ischemia induces podocyte effacement with loss of slit diaphragm and proteinuria. Biochemical analysis of the ischemic glomerulus shows that ischemia induces rapid loss of interaction between slit diaphragm junctional proteins Neph1 and ZO-1. To further understand the effect of ischemia on molecular interactions between slit diaphragm proteins, a cell culture model was employed to study th2008-01-01T00:00:00Z2008 Dec2025-04-26T00:31:12.007Z2019-03-27T00:19:48ZS-EPMC26028821892280110.1074/jbc.M80550720010.1074/jbc.m805507200