biostudies-literatureKalabis JNIDDK NIH HHSNCI NIH HHSG1182-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC2604801biostudies-literatureUnknown295(6)Cells within the normal human colonic epithelium undergo a dynamic cycle of growth, differentiation, and death. The organotypic culture system of human fetal colonic epithelial cells seeded on top of collagen gels with embedded colonic fibroblasts allowed prolonged culture of the colonic epithelial cells (Kalabis J, Patterson MJ, Enders GM, Marian B, Iozzo RV, Rogler G, Gimotty PA, Herlyn M. FASEB J 17: 1115-1117, 2003). Herein, we have evaluated the role of endothelin-3 (ET3) and both cognate endothelin receptors (ETRA, ETRB) for human colonic epithelial cell growth and survival. ET3 was produced continuously by the fibroblasts as a result of adenovirus-mediated gene transfer. The presence and function of the endothelin receptors (ETRs) in epithelial cells was evaluated by [(3)H]thymidineAmerican journal of physiology. Gastrointestinal and liver physiologyEndothelin-3 stimulates survival of goblet cells in organotypic cultures of fetal human colonic epithelium.PMC2604801CA-25874DK-056645CA-74294Li GHerlyn MKalabis JFukunaga-Kalabis MRustgi AKfalseEndothelin-3 stimulates survival of goblet cells in organotypic cultures of fetal human colonic epithelium.Cells within the normal human colonic epithelium undergo a dynamic cycle of growth, differentiation, and death. The organotypic culture system of human fetal colonic epithelial cells seeded on top of collagen gels with embedded colonic fibroblasts allowed prolonged culture of the colonic epithelial cells (Kalabis J, Patterson MJ, Enders GM, Marian B, Iozzo RV, Rogler G, Gimotty PA, Herlyn M. FASEB J 17: 1115-1117, 2003). Herein, we have evaluated the role of endothelin-3 (ET3) and both cognate endothelin receptors (ETRA, ETRB) for human colonic epithelial cell growth and survival. ET3 was produced continuously by the fibroblasts as a result of adenovirus-mediated gene transfer. The presence and function of the endothelin receptors (ETRs) in epithelial cells was evaluated by [(3)H]thymidine2008-01-01T00:00:00Z2008 Dec2025-04-26T00:34:14.049Z2019-03-27T00:19:50ZS-EPMC26048011883245010.1152/ajpgi.90294.2008