{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rosker C"],"funding":["Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["5186-94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2643496"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["284(8)"],"pubmed_abstract":["Ryanodine receptors (RyR) are Ca(2+) channels that mediate Ca(2+) release from intracellular stores in response to diverse intracellular signals. In RINm5F insulinoma cells, caffeine, and 4-chloro-m-cresol (4CmC), agonists of RyR, stimulated Ca(2+) entry that was independent of store-operated Ca(2+) entry, and blocked by prior incubation with a concentration of ryanodine that inactivates RyR. Patch-clamp recording identified small numbers of large-conductance (gamma(K) = 169 pS) cation channels that were activated by caffeine, 4CmC or low concentrations of ryanodine. Similar channels were detected in rat pancreatic beta-cells. In RINm5F cells, the channels were blocked by cytosolic, but not extracellular, ruthenium red. Subcellular fractionation showed that type 3 IP(3) receptors (IP(3)R3)"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Functional ryanodine receptors in the plasma membrane of RINm5F pancreatic beta-cells."],"pmcid":["PMC2643496"],"funding_grant_id":["BB/E004660/1"],"pubmed_authors":["Rosker C","Taylor CW","Taylor EJ","Meur G"],"additional_accession":[]},"is_claimable":false,"name":"Functional ryanodine receptors in the plasma membrane of RINm5F pancreatic beta-cells.","description":"Ryanodine receptors (RyR) are Ca(2+) channels that mediate Ca(2+) release from intracellular stores in response to diverse intracellular signals. In RINm5F insulinoma cells, caffeine, and 4-chloro-m-cresol (4CmC), agonists of RyR, stimulated Ca(2+) entry that was independent of store-operated Ca(2+) entry, and blocked by prior incubation with a concentration of ryanodine that inactivates RyR. Patch-clamp recording identified small numbers of large-conductance (gamma(K) = 169 pS) cation channels that were activated by caffeine, 4CmC or low concentrations of ryanodine. Similar channels were detected in rat pancreatic beta-cells. In RINm5F cells, the channels were blocked by cytosolic, but not extracellular, ruthenium red. Subcellular fractionation showed that type 3 IP(3) receptors (IP(3)R3)","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Feb","modification":"2026-03-16T16:43:57.86Z","creation":"2019-03-27T00:20:46Z"},"accession":"S-EPMC2643496","cross_references":{"pubmed":["19116207"],"doi":["10.1074/jbc.M805587200"]}}