{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Goc A"],"funding":["NEI NIH HHS","NIGMS NIH HHS"],"pagination":["12409-19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2645919"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(47)"],"pubmed_abstract":["Visual signal transduction serves as one of the best understood G protein-coupled receptor signaling systems. Signaling is initiated when a photon strikes rhodopsin (Rho) causing a conformational change leading to productive interaction of this G protein-coupled receptor with the heterotrimeric G protein, transducin (Gt). Here we describe a new method for Gt purification from native bovine rod photoreceptor membranes without subunit dissociation caused by exposure to photoactivated rhodopsin (Rho*). Native electrophoresis followed by immunoblotting revealed that Gt purified by this method formed more stable heterotrimers and interacted more efficiently with membranes containing Rho* or its target, phosphodiesterase 6, than did Gt purified by a traditional method involving subunit dissociat"],"journal":["Biochemistry"],"pubmed_title":["Different properties of the native and reconstituted heterotrimeric G protein transducin."],"pmcid":["PMC2645919"],"funding_grant_id":["GM079191","P30 EY11373","P30 EY011373","R01 EY008061","EY008061","R01 GM079191","P30 EY011373-129005","R01 EY008061-22","R01 GM079191-02","T32 EY007157"],"pubmed_authors":["Goc A","Palczewski K","Wang B","Jastrzebska B","Angel TE","Wintrode PL"],"additional_accession":[]},"is_claimable":false,"name":"Different properties of the native and reconstituted heterotrimeric G protein transducin.","description":"Visual signal transduction serves as one of the best understood G protein-coupled receptor signaling systems. Signaling is initiated when a photon strikes rhodopsin (Rho) causing a conformational change leading to productive interaction of this G protein-coupled receptor with the heterotrimeric G protein, transducin (Gt). Here we describe a new method for Gt purification from native bovine rod photoreceptor membranes without subunit dissociation caused by exposure to photoactivated rhodopsin (Rho*). Native electrophoresis followed by immunoblotting revealed that Gt purified by this method formed more stable heterotrimers and interacted more efficiently with membranes containing Rho* or its target, phosphodiesterase 6, than did Gt purified by a traditional method involving subunit dissociat","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Nov","modification":"2025-04-04T07:58:30.842Z","creation":"2019-03-27T00:20:50Z"},"accession":"S-EPMC2645919","cross_references":{"pubmed":["18975915"],"doi":["10.1021/bi8015444"]}}