biostudies-literatureGoc ANEI NIH HHSNIGMS NIH HHS12409-19https://www.ebi.ac.uk/biostudies/studies/S-EPMC2645919biostudies-literatureUnknown47(47)Visual signal transduction serves as one of the best understood G protein-coupled receptor signaling systems. Signaling is initiated when a photon strikes rhodopsin (Rho) causing a conformational change leading to productive interaction of this G protein-coupled receptor with the heterotrimeric G protein, transducin (Gt). Here we describe a new method for Gt purification from native bovine rod photoreceptor membranes without subunit dissociation caused by exposure to photoactivated rhodopsin (Rho*). Native electrophoresis followed by immunoblotting revealed that Gt purified by this method formed more stable heterotrimers and interacted more efficiently with membranes containing Rho* or its target, phosphodiesterase 6, than did Gt purified by a traditional method involving subunit dissociatBiochemistryDifferent properties of the native and reconstituted heterotrimeric G protein transducin.PMC2645919GM079191P30 EY11373P30 EY011373R01 EY008061EY008061R01 GM079191P30 EY011373-129005R01 EY008061-22R01 GM079191-02T32 EY007157Goc APalczewski KWang BJastrzebska BAngel TEWintrode PLfalseDifferent properties of the native and reconstituted heterotrimeric G protein transducin.Visual signal transduction serves as one of the best understood G protein-coupled receptor signaling systems. Signaling is initiated when a photon strikes rhodopsin (Rho) causing a conformational change leading to productive interaction of this G protein-coupled receptor with the heterotrimeric G protein, transducin (Gt). Here we describe a new method for Gt purification from native bovine rod photoreceptor membranes without subunit dissociation caused by exposure to photoactivated rhodopsin (Rho*). Native electrophoresis followed by immunoblotting revealed that Gt purified by this method formed more stable heterotrimers and interacted more efficiently with membranes containing Rho* or its target, phosphodiesterase 6, than did Gt purified by a traditional method involving subunit dissociat2008-01-01T00:00:00Z2008 Nov2025-04-04T07:58:30.842Z2019-03-27T00:20:50ZS-EPMC26459191897591510.1021/bi8015444