biostudies-literatureBora ANIDA NIH HHS4992-5003https://www.ebi.ac.uk/biostudies/studies/S-EPMC2646869biostudies-literatureUnknown7(11)The mammalian supraoptic nucleus (SON) is a neuroendocrine center in the brain regulating a variety of physiological functions. Within the SON, peptidergic magnocellular neurons that project to the neurohypophysis (posterior pituitary) are involved in controlling osmotic balance, lactation, and parturition, partly through secretion of signaling peptides such as oxytocin and vasopressin into the blood. An improved understanding of SON activity and function requires identification and characterization of the peptides used by the SON. Here, small-volume sample preparation approaches are optimized for neuropeptidomic studies of isolated SON samples ranging from entire nuclei down to single magnocellular neurons. Unlike most previous mammalian peptidome studies, tissues are not immediately heatJournal of proteome researchNeuropeptidomics of the supraoptic rat nucleus.PMC2646869DA018310DA017940R01 DA017940P30 DA018310Millet LJAnnangudi SPGillette MUBora AForbes AJKelleher NLSweedler JVRubakhin SSfalseNeuropeptidomics of the supraoptic rat nucleus.The mammalian supraoptic nucleus (SON) is a neuroendocrine center in the brain regulating a variety of physiological functions. Within the SON, peptidergic magnocellular neurons that project to the neurohypophysis (posterior pituitary) are involved in controlling osmotic balance, lactation, and parturition, partly through secretion of signaling peptides such as oxytocin and vasopressin into the blood. An improved understanding of SON activity and function requires identification and characterization of the peptides used by the SON. Here, small-volume sample preparation approaches are optimized for neuropeptidomic studies of isolated SON samples ranging from entire nuclei down to single magnocellular neurons. Unlike most previous mammalian peptidome studies, tissues are not immediately heat2008-01-01T00:00:00Z2008 Nov2025-04-04T07:58:18.578Z2019-03-27T00:20:52ZS-EPMC26468691881608510.1021/pr800394e