biostudies-literaturePolati RTelethon4https://www.ebi.ac.uk/biostudies/studies/S-EPMC2649903biostudies-literatureUnknown7<h4>Background</h4>Macrophages are involved in a number of key physiological processes and complex responses such as inflammatory, immunological, infectious diseases and iron homeostasis. These cells are specialised for iron storage and recycling from senescent erythrocytes so they play a central role in the fine tuning of iron balancing and distribution. The comprehension of the many physiological responses of macrophages implies the study of the related molecular events. To this regard, proteomic analysis, is one of the most powerful tools for the elucidation of the molecular mechanisms, in terms of changes in protein expression levels.<h4>Results</h4>Our aim was to optimize a protocol for protein fractionation and high resolution mapping using human macrophages for clinical studies. We Proteome scienceHigh resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics.PMC2649903GGP06213Polati RBossi AGirelli DZolla LTimperio AMOlivieri OCorrocher RCampostrini NCastagna AZaninotto FfalseHigh resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics.<h4>Background</h4>Macrophages are involved in a number of key physiological processes and complex responses such as inflammatory, immunological, infectious diseases and iron homeostasis. These cells are specialised for iron storage and recycling from senescent erythrocytes so they play a central role in the fine tuning of iron balancing and distribution. The comprehension of the many physiological responses of macrophages implies the study of the related molecular events. To this regard, proteomic analysis, is one of the most powerful tools for the elucidation of the molecular mechanisms, in terms of changes in protein expression levels.<h4>Results</h4>Our aim was to optimize a protocol for protein fractionation and high resolution mapping using human macrophages for clinical studies. We 2009-01-01T00:00:00Z2009 Feb2025-04-04T13:39:47.634Z2019-03-27T00:20:58ZS-EPMC26499031922839910.1186/1477-5956-7-4