biostudies-literature00620Bajaj CLNIBIB NIH HHSNCRR NIH HHSNIGMS NIH HHS236-239https://www.ebi.ac.uk/biostudies/studies/S-EPMC2678009biostudies-literatureUnknown2007Three dimensional Electron Microscopy (EM) and in particular single particle reconstruction using cryo-EM, has rapidly advanced over recent years, such that increasingly several macromolecular complexes can be resolved at subnanometer resolution (6-10 Å). This paper reviews some of the main volumetric image and geometric post-processing steps once a three dimensional EM map (henceforth a 3D map) has been reconstructed from single particle Cryo-EM, as essential steps in an enhanced and automated computational structure interpretation pipeline. In particular the paper addresses automated filtering, critical point calculations, symmetric and non-symmetric molecular domain segmentation, molecular surface selection, curation, and protein secondary structure (α- helices and β-sheets) elucidation from 3D maps.Proceedings. IEEE International Symposium on Biomedical ImagingAUTOMATIC STRUCTURE INTERPRETATION OF SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY: FROM IMAGES TO PSUEDO-ATOMIC MODELS.PMC2678009P41 RR008605-147784R01 EB004873-01A2R01 GM073087-01A1P41 RR002250-200095R01 GM074258-02R01 GM073087R01 GM074258-03P41 RR008605-147779P41 RR008605-158490R01 GM073087-03P41 RR008605R01 GM073087-02R01 GM074258-01P41 RR002250-217415P41 RR008605-158485P41 RR002250R01 GM074258P41 RR002250-226519R01 EB004873R01 EB004873-02P20 RR020647Bajaj CL62falseAUTOMATIC STRUCTURE INTERPRETATION OF SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY: FROM IMAGES TO PSUEDO-ATOMIC MODELS.Three dimensional Electron Microscopy (EM) and in particular single particle reconstruction using cryo-EM, has rapidly advanced over recent years, such that increasingly several macromolecular complexes can be resolved at subnanometer resolution (6-10 Å). This paper reviews some of the main volumetric image and geometric post-processing steps once a three dimensional EM map (henceforth a 3D map) has been reconstructed from single particle Cryo-EM, as essential steps in an enhanced and automated computational structure interpretation pipeline. In particular the paper addresses automated filtering, critical point calculations, symmetric and non-symmetric molecular domain segmentation, molecular surface selection, curation, and protein secondary structure (α- helices and β-sheets) elucidation from 3D maps.2007-01-01T00:00:00Z2007 Apr2024-10-15T16:43:42.593Z2019-03-27T00:22:11ZS-EPMC26780091942445510.1109/isbi.2007.35683210.1109/ISBI.2007.356832