biostudies-literatureDuncan CGNCI NIH HHSNINDS NIH HHS22https://www.ebi.ac.uk/biostudies/studies/S-EPMC2685141biostudies-literatureUnknown2<h4>Background</h4>Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered.<h4>Methods</h4>We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library.<h4>Results</h4>To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of micrBMC medical genomicsIdentification of microbial DNA in human cancer.PMC2685141P50 CA108786R01 NS052507R01 CA121113P20 CA096890R01 CA118822P50 NS020023P30 CA014236R37 CA011898Lin JCBigner DKinzler KWDi CEdwards JDuncan CGRiggins GJVelculescu VECummins JWang TLVogelstein BKopelovich LLeary RJYan HSchaefer CFfalseIdentification of microbial DNA in human cancer.<h4>Background</h4>Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered.<h4>Methods</h4>We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library.<h4>Results</h4>To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of micr2009-01-01T00:00:00Z2009 May2025-05-29T21:51:57.908Z2025-05-29T21:51:57.908ZS-EPMC26851411942650510.1186/1755-8794-2-22