{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3"],"submitter":["Felske AD"],"pubmed_abstract":["Background
Bacterial mercury resistance is based on enzymatic reduction of ionic mercury to elemental mercury and has recently been demonstrated to be applicable for industrial wastewater clean-up. The long-term monitoring of such biocatalyser systems requires a cultivation independent functional community profiling method targeting the key enzyme of the process, the merA gene coding for the mercuric reductase. We report on the development of a profiling method for merA and its application to monitor changes in the functional diversity of the biofilm community of a technical scale biocatalyzer over 8 months of on-site operation.Results
Based on an alignment of 30 merA sequences from Gram negative bacteria, conserved primers were designed for amplification of merA fragments "],"journal":["BMC microbiology"],"pagination":["22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC270059"],"repository":["biostudies-literature"],"pubmed_title":["Functional profiling of mercuric reductase (mer A) genes in biofilm communities of a technical scale biocatalyzer."],"pmcid":["PMC270059"],"pubmed_authors":["von Canstein H","Fehr W","Wagner-Dobler I","Pauling BV","Felske AD"],"additional_accession":[]},"is_claimable":false,"name":"Functional profiling of mercuric reductase (mer A) genes in biofilm communities of a technical scale biocatalyzer.","description":"Background
Bacterial mercury resistance is based on enzymatic reduction of ionic mercury to elemental mercury and has recently been demonstrated to be applicable for industrial wastewater clean-up. The long-term monitoring of such biocatalyser systems requires a cultivation independent functional community profiling method targeting the key enzyme of the process, the merA gene coding for the mercuric reductase. We report on the development of a profiling method for merA and its application to monitor changes in the functional diversity of the biofilm community of a technical scale biocatalyzer over 8 months of on-site operation.Results
Based on an alignment of 30 merA sequences from Gram negative bacteria, conserved primers were designed for amplification of merA fragments ","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Oct","modification":"2025-05-29T21:53:45.359Z","creation":"2019-03-27T00:35:59Z"},"accession":"S-EPMC270059","cross_references":{"pubmed":["14577839"],"doi":["10.1186/1471-2180-3-22"]}}