biostudies-literatureUnknown8(7)Yu YPreimplantation genetic diagnosis (PGD), used in clinical practice, is offered to couples that may suffer from a monogenetic disorder, chromosome aneuploidy, or X-linked disease. However, blastomere biopsy, as an indispensable manipulation during the PGD procedure has not been assessed for its long term health implications. Using a mouse model, we investigated the effect of blastomere biopsy of in vitro cultured four-cell embryos on preimplantation development efficiency, postnatal growth, and physiological and behavioral activity compared with control, non-biopsied embryos. The mice generated after blastomere biopsy showed weight increase and some memory decline compared with the control group. Further protein expression profiles in adult brains were analyzed by a proteomics approach. A tMolecular & cellular proteomics : MCP1490-500https://www.ebi.ac.uk/biostudies/studies/S-EPMC2709181biostudies-literatureEvaluation of blastomere biopsy using a mouse model indicates the potential high risk of neurodegenerative disorders in the offspring.PMC2709181Lv ZWu JLiu JFan YZhao CZhou QGuo XZhou RChen LWang FZhu HZhou ZWang LXiao MSha JZhang ZChen WHuo RLin MYu YfalseEvaluation of blastomere biopsy using a mouse model indicates the potential high risk of neurodegenerative disorders in the offspring.Preimplantation genetic diagnosis (PGD), used in clinical practice, is offered to couples that may suffer from a monogenetic disorder, chromosome aneuploidy, or X-linked disease. However, blastomere biopsy, as an indispensable manipulation during the PGD procedure has not been assessed for its long term health implications. Using a mouse model, we investigated the effect of blastomere biopsy of in vitro cultured four-cell embryos on preimplantation development efficiency, postnatal growth, and physiological and behavioral activity compared with control, non-biopsied embryos. The mice generated after blastomere biopsy showed weight increase and some memory decline compared with the control group. Further protein expression profiles in adult brains were analyzed by a proteomics approach. A t2009-01-01T00:00:00Z2009 Jul2025-04-04T14:37:36.907Z2019-06-06T21:31:06ZS-EPMC27091811927904310.1074/mcp.M800273-MCP20010.1074/mcp.m800273-mcp200