biostudies-literatureUnknown8(7)Doro FSafe recombinant vaccines, based on a small number of antigenic proteins, are emerging as the most attractive, cost-effective solution against infectious diseases. In the present work, we confirmed previous data from our laboratory showing that whole viable bacterial cell treatment with proteases followed by the identification of released peptides by mass spectrometry is the method of choice for the rapid and reliable identification of vaccine candidates in Gram-positive bacteria. When applied to the Group B Streptococcus COH1 strain, 43 surface-associated proteins were identified, including all the protective antigens described in the literature as well as a new protective antigen, the cell wall-anchored protein SAN_1485 belonging to the serine-rich repeat protein family. This strategy ovMolecular & cellular proteomics : MCP1728-37https://www.ebi.ac.uk/biostudies/studies/S-EPMC2709197biostudies-literatureSurfome analysis as a fast track to vaccine discovery: identification of a novel protective antigen for Group B Streptococcus hypervirulent strain COH1.PMC2709197Margarit IRodriguez-Ortega MJMaione DAltindis EMora MScarselli MNorais NRosini RD'Aurizio RDoro FStella MLiberatori SGrandi GRinaudo CDTelford JLfalseSurfome analysis as a fast track to vaccine discovery: identification of a novel protective antigen for Group B Streptococcus hypervirulent strain COH1.Safe recombinant vaccines, based on a small number of antigenic proteins, are emerging as the most attractive, cost-effective solution against infectious diseases. In the present work, we confirmed previous data from our laboratory showing that whole viable bacterial cell treatment with proteases followed by the identification of released peptides by mass spectrometry is the method of choice for the rapid and reliable identification of vaccine candidates in Gram-positive bacteria. When applied to the Group B Streptococcus COH1 strain, 43 surface-associated proteins were identified, including all the protective antigens described in the literature as well as a new protective antigen, the cell wall-anchored protein SAN_1485 belonging to the serine-rich repeat protein family. This strategy ov2009-01-01T00:00:00Z2009 Jul2025-04-04T14:37:38.429Z2019-06-06T21:31:07ZS-EPMC27091971940159710.1074/mcp.M800486-MCP20010.1074/mcp.m800486-mcp200