{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["119(8)"],"submitter":["Alvarez-Diaz S"],"pubmed_abstract":["The active vitamin D metabolite 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] has wide but not fully understood antitumor activity. A previous transcriptomic analysis of 1alpha,25(OH)2D3 action on human colon cancer cells revealed cystatin D (CST5), which encodes an inhibitor of several cysteine proteases of the cathepsin family, as a candidate target gene. Here we report that 1alpha,25(OH)2D3 induced vitamin D receptor (VDR) binding to, and activation of, the CST5 promoter and increased CST5 RNA and protein levels in human colon cancer cells. In cells lacking endogenous cystatin D, ectopic cystatin D expression inhibited both proliferation in vitro and xenograft tumor growth in vivo. Furthermore, cystatin D inhibited migration and anchorage-independent growth, antagonized the Wnt/beta-"],"journal":["The Journal of clinical investigation"],"pagination":["2343-58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2719930"],"repository":["biostudies-literature"],"pubmed_title":["Cystatin D is a candidate tumor suppressor  gene induced by vitamin D in human  colon cancer cells."],"pmcid":["PMC2719930"],"pubmed_authors":["Astudillo A","Alvarez-Diaz S","Garcia JM","Valle N","Freije JM","Bonilla F","Lopez-Otin C","Quesada V","Munoz A","Pena C"],"additional_accession":[]},"is_claimable":false,"name":"Cystatin D is a candidate tumor suppressor  gene induced by vitamin D in human  colon cancer cells.","description":"The active vitamin D metabolite 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] has wide but not fully understood antitumor activity. A previous transcriptomic analysis of 1alpha,25(OH)2D3 action on human colon cancer cells revealed cystatin D (CST5), which encodes an inhibitor of several cysteine proteases of the cathepsin family, as a candidate target gene. Here we report that 1alpha,25(OH)2D3 induced vitamin D receptor (VDR) binding to, and activation of, the CST5 promoter and increased CST5 RNA and protein levels in human colon cancer cells. In cells lacking endogenous cystatin D, ectopic cystatin D expression inhibited both proliferation in vitro and xenograft tumor growth in vivo. Furthermore, cystatin D inhibited migration and anchorage-independent growth, antagonized the Wnt/beta-","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Aug","modification":"2025-04-19T05:36:19.484Z","creation":"2019-03-27T00:23:56Z"},"accession":"S-EPMC2719930","cross_references":{"pubmed":["19662683"],"doi":["10.1172/jci37205"]}}