{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Klechevsky E"],"funding":["NIAID NIH HHS","NCI NIH HHS"],"pagination":["6360-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2728007"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["68(15)"],"pubmed_abstract":["In this study, we have explored the use of Fab-toxin proteins (immunotoxin) to target antigen-specific MHC-peptide complexes of in vitro and in vivo cancer cells. A human phage display library was used to screen for T-cell receptor (TCR)-like antibodies that are highly specific for the peptide melanoma-associated antigen MART-1(26-35) presented by HLA-A201. We also used previously selected TCR-like antibodies specific for the peptide melanoma-associated antigen gp100(280-288) presented by HLA-A201. The recombinant immunotoxin constructs were generated by fusing the targeting Fab fragment to a truncated form of Pseudomonas exotoxin, PE38KDEL. These immunotoxins bound with high affinity to the EBV-transformed JY cell line pulsed with the aforementioned peptides and internalized within 30 min"],"journal":["Cancer research"],"pubmed_title":["Antitumor activity of immunotoxins with T-cell receptor-like specificity against human melanoma xenografts."],"pmcid":["PMC2728007"],"funding_grant_id":["R0-1 CA78846","P01 CA084512-069004","P01 CA084512-069002","P01 CA084512-01A10003","P0-1 CA84512","U19 AI057234-03","U19 AI057234-04","U19 AI057234-05","R0-1 CA85540","U19 AI057234-01","U19 AI057234-02","U19 AI057234-010005","U19 AI057234-010004","P01 CA084512-020003","R01 CA085540-02","R01 CA085540-03","R01 CA085540-04","R0-1 CA115550","U19 AI057234-05S19003","R01 CA115550-03","R01 CA115550-04","R01 CA115550-01","R01 CA115550-02","U19 AI057234-05S1","U19 AI057234-05S10012","U19 AI057234","R01 CA078846","U-19 AI-57234","R01 CA078846-10","P01 CA084512","P01 CA084512-040003","P01 CA084512-060005","P01 CA084512-05A29004","P01 CA084512-05A29002","R01 CA078846-01A1","P01 CA084512-05A2","R01 CA115550","P01 CA084512-07","P01 CA084512-030003","P01 CA084512-06","R01 CA078846-09","R01 CA078846-08","R01 CA078846-07","P01 CA084512-070005","R01 CA078846-06","R01 CA078846-05","P01 CA084512-05A20005","R01 CA078846-04","R01 CA078846-03","R01 CA078846-02","R01 CA085540","P01 CA084512-079002","R01 CA085540-01A1","P01 CA084512-079004"],"pubmed_authors":["Denkberg G","Reiter Y","Klechevsky E","Gallegos M","Palucka K","Cohen C","Banchereau J"],"additional_accession":[]},"is_claimable":false,"name":"Antitumor activity of immunotoxins with T-cell receptor-like specificity against human melanoma xenografts.","description":"In this study, we have explored the use of Fab-toxin proteins (immunotoxin) to target antigen-specific MHC-peptide complexes of in vitro and in vivo cancer cells. A human phage display library was used to screen for T-cell receptor (TCR)-like antibodies that are highly specific for the peptide melanoma-associated antigen MART-1(26-35) presented by HLA-A201. We also used previously selected TCR-like antibodies specific for the peptide melanoma-associated antigen gp100(280-288) presented by HLA-A201. The recombinant immunotoxin constructs were generated by fusing the targeting Fab fragment to a truncated form of Pseudomonas exotoxin, PE38KDEL. These immunotoxins bound with high affinity to the EBV-transformed JY cell line pulsed with the aforementioned peptides and internalized within 30 min","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Aug","modification":"2025-04-04T09:10:34.851Z","creation":"2019-03-27T00:24:18Z"},"accession":"S-EPMC2728007","cross_references":{"pubmed":["18676861"],"doi":["10.1158/0008-5472.can-08-0928","10.1158/0008-5472.CAN-08-0928"]}}