{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Antos JM"],"funding":["NIBIB NIH HHS","NIAID NIH HHS"],"pagination":["10800-1"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2730784"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["131(31)"],"pubmed_abstract":["The unique reactivity of two sortase enzymes, SrtA(staph) from Staphylococcus aureus and SrtA(strep) from Streptococcus pyogenes, is exploited for site-specific labeling of a single polypeptide with different labels at its N and C termini. SrtA(strep) is used to label the protein's C terminus at an LPXTG site with a fluorescently labeled dialanine nucleophile. Selective N-terminal labeling of proteins containing N-terminal glycine residues is achieved using SrtA(staph) and LPXT derivatives. The generality of N-terminal labeling with SrtA(staph) is demonstrated by near-quantitative labeling of multiple protein substrates with excellent site specificity."],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity."],"pmcid":["PMC2730784"],"funding_grant_id":["R01-AI057182","R01-AI033456","R21 EB008875","R01 AI057182","R21-EB008875","R01 AI033456"],"pubmed_authors":["Chew GL","Popp MW","Antos JM","Grotenbreg GM","Ploegh HL","Guimaraes CP","Yoder NC"],"additional_accession":[]},"is_claimable":false,"name":"Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity.","description":"The unique reactivity of two sortase enzymes, SrtA(staph) from Staphylococcus aureus and SrtA(strep) from Streptococcus pyogenes, is exploited for site-specific labeling of a single polypeptide with different labels at its N and C termini. SrtA(strep) is used to label the protein's C terminus at an LPXTG site with a fluorescently labeled dialanine nucleophile. Selective N-terminal labeling of proteins containing N-terminal glycine residues is achieved using SrtA(staph) and LPXT derivatives. The generality of N-terminal labeling with SrtA(staph) is demonstrated by near-quantitative labeling of multiple protein substrates with excellent site specificity.","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Aug","modification":"2025-04-05T11:53:09.076Z","creation":"2019-03-27T00:24:25Z"},"accession":"S-EPMC2730784","cross_references":{"pubmed":["19610623"],"doi":["10.1021/ja902681k"]}}