biostudies-literatureHe GNIGMS NIH HHS12088-90https://www.ebi.ac.uk/biostudies/studies/S-EPMC2742410biostudies-literatureUnknown131(34)In this communication, we show that peptide nucleic acids (PNAs) with lengths of 15-20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson-Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of gammaPNA is required for invasion in this case, and no nucleobase substitution is needed.Journal of the American Chemical SocietyStrand invasion of extended, mixed-sequence B-DNA by gammaPNAs.PMC2742410R01 GM077261R01 GM077261-04GM076251Rapireddy SLy DHHe GSahu BBahal RfalseStrand invasion of extended, mixed-sequence B-DNA by gammaPNAs.In this communication, we show that peptide nucleic acids (PNAs) with lengths of 15-20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson-Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of gammaPNA is required for invasion in this case, and no nucleobase substitution is needed.2009-01-01T00:00:00Z2009 Sep2025-04-20T01:51:50.65Z2019-03-27T00:24:52ZS-EPMC27424101966342410.1021/ja900228j