{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["284(31)"],"submitter":["Tallack MR"],"pubmed_abstract":["Differentiation of erythroid cells requires precise control over the cell cycle to regulate the balance between cell proliferation and differentiation. The zinc finger transcription factor, erythroid Krüppel-like factor (EKLF/KLF1), is essential for proper erythroid cell differentiation and regulates many erythroid genes. Here we show that loss of EKLF leads to aberrant entry into S-phase of the cell cycle during both primitive and definitive erythropoiesis. This cell cycle defect was associated with a significant reduction in the expression levels of E2f2 and E2f4, key factors necessary for the induction of S-phase gene expression and erythropoiesis. We found and validated novel intronic enhancers in both the E2f2 and E2f4 genes, which contain conserved CACC, GATA, and E-BOX elements. The"],"journal":["The Journal of biological chemistry"],"pagination":["20966-74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2742862"],"repository":["biostudies-literature"],"pubmed_title":["EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2."],"pmcid":["PMC2742862"],"pubmed_authors":["Perkins AC","Tallack MR","Humbert PO","Keys JR"],"additional_accession":[]},"is_claimable":false,"name":"EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2.","description":"Differentiation of erythroid cells requires precise control over the cell cycle to regulate the balance between cell proliferation and differentiation. The zinc finger transcription factor, erythroid Krüppel-like factor (EKLF/KLF1), is essential for proper erythroid cell differentiation and regulates many erythroid genes. Here we show that loss of EKLF leads to aberrant entry into S-phase of the cell cycle during both primitive and definitive erythropoiesis. This cell cycle defect was associated with a significant reduction in the expression levels of E2f2 and E2f4, key factors necessary for the induction of S-phase gene expression and erythropoiesis. We found and validated novel intronic enhancers in both the E2f2 and E2f4 genes, which contain conserved CACC, GATA, and E-BOX elements. The","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Jul","modification":"2025-04-20T01:54:29.548Z","creation":"2019-03-27T00:24:55Z"},"accession":"S-EPMC2742862","cross_references":{"pubmed":["19457859"],"doi":["10.1074/jbc.m109.006346","10.1074/jbc.M109.006346"]}}