{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(18)"],"submitter":["Rotem A"],"pubmed_abstract":["The nuclear envelope of higher eukaryotic cells reforms at the exit from mitosis, in concert with the assembly of nuclear pore complexes (NPCs). The first step in postmitotic NPC assembly involves the \"seeding\" of chromatin with ELYS and the Nup107-160 complex. Subsequent steps in the assembly process are poorly understood and different mechanistic models have been proposed to explain the formation of the full supramolecular structure. Here, we show that the initial step of chromatin seeding is negatively regulated by importin beta. Direct imaging of the chromatin attachment sites reveals single sites situated predominantly on the highest substructures of chromatin surface and lacking any sign of annular structures or oligomerized pre-NPCs. Surprisingly, the inhibition by importin beta is "],"journal":["Molecular biology of the cell"],"pagination":["4031-42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2743622"],"repository":["biostudies-literature"],"pubmed_title":["Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly."],"pmcid":["PMC2743622"],"pubmed_authors":["Klein E","Gruber R","Shorer H","Shaulov L","Rotem A","Harel A"],"additional_accession":[]},"is_claimable":false,"name":"Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly.","description":"The nuclear envelope of higher eukaryotic cells reforms at the exit from mitosis, in concert with the assembly of nuclear pore complexes (NPCs). The first step in postmitotic NPC assembly involves the \"seeding\" of chromatin with ELYS and the Nup107-160 complex. Subsequent steps in the assembly process are poorly understood and different mechanistic models have been proposed to explain the formation of the full supramolecular structure. Here, we show that the initial step of chromatin seeding is negatively regulated by importin beta. Direct imaging of the chromatin attachment sites reveals single sites situated predominantly on the highest substructures of chromatin surface and lacking any sign of annular structures or oligomerized pre-NPCs. Surprisingly, the inhibition by importin beta is ","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Sep","modification":"2025-04-26T14:35:15.006Z","creation":"2019-03-27T00:24:58Z"},"accession":"S-EPMC2743622","cross_references":{"pubmed":["19625448"],"doi":["10.1091/mbc.e09-02-0150"]}}