biostudies-literatureDrosatos KNHLBI NIH HHS19556-64https://www.ebi.ac.uk/biostudies/studies/S-EPMC2745720biostudies-literatureUnknown282(27)c-Jun is a transcription factor activated by phosphorylation by the stress-activated protein kinase/c-Jun N-terminal kinase pathway in response to extracellular signals and cytokines. We show that adenovirus-mediated gene transfer of the dominant negative form of c-Jun (dn-c-Jun) in C57BL/6 mice increased greatly apoE hepatic mRNA and plasma levels, increased plasma cholesterol, triglyceride, and very low density lipoprotein levels, and resulted in the accumulation of discoidal high density lipoprotein particles. A similar but more severe phenotype was generated by overexpression of the mouse apoE in C57BL/6 mice, suggesting that dyslipidemia induced by dn-c-Jun was the result of apoE overexpression. Unexpectedly, infection of apoE(-/-) mice with adenovirus expressing dn-c-Jun reduced plasThe Journal of biological chemistryA dominant negative form of the transcription factor c-Jun affects genes that have opposing effects on lipid homeostasis in mice.PMC2745720HL33952R01 HL033952R01 HL048739R01 HL068216HL48739Kypreos KEDrosatos KKardassis DSanoudou DZannis VIfalseA dominant negative form of the transcription factor c-Jun affects genes that have opposing effects on lipid homeostasis in mice.c-Jun is a transcription factor activated by phosphorylation by the stress-activated protein kinase/c-Jun N-terminal kinase pathway in response to extracellular signals and cytokines. We show that adenovirus-mediated gene transfer of the dominant negative form of c-Jun (dn-c-Jun) in C57BL/6 mice increased greatly apoE hepatic mRNA and plasma levels, increased plasma cholesterol, triglyceride, and very low density lipoprotein levels, and resulted in the accumulation of discoidal high density lipoprotein particles. A similar but more severe phenotype was generated by overexpression of the mouse apoE in C57BL/6 mice, suggesting that dyslipidemia induced by dn-c-Jun was the result of apoE overexpression. Unexpectedly, infection of apoE(-/-) mice with adenovirus expressing dn-c-Jun reduced plas2007-01-01T00:00:00Z2007 Jul2026-05-02T05:40:50.93Z2026-04-07T17:42:06.129ZS-EPMC27457201745646710.1074/jbc.M70098620010.1074/jbc.m700986200