{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ko J"],"funding":["NIMH NIH HHS"],"pagination":["3244-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2771087"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(20)"],"pubmed_abstract":["Postsynaptic neuroligins are thought to perform essential functions in synapse validation and synaptic transmission by binding to, and dimerizing, presynaptic alpha- and beta-neurexins. To test this hypothesis, we examined the functional effects of neuroligin-1 mutations that impair only alpha-neurexin binding, block both alpha- and beta-neurexin binding, or abolish neuroligin-1 dimerization. Abolishing alpha-neurexin binding abrogated neuroligin-induced generation of neuronal synapses onto transfected non-neuronal cells in the so-called artificial synapse-formation assay, even though beta-neurexin binding was retained. Thus, in this assay, neuroligin-1 induces apparent synapse formation by binding to presynaptic alpha-neurexins. In transfected neurons, however, neither alpha- nor beta-neu"],"journal":["The EMBO journal"],"pubmed_title":["Neuroligin-1 performs neurexin-dependent and neurexin-independent functions in synapse validation."],"pmcid":["PMC2771087"],"funding_grant_id":["R37 MH052804"],"pubmed_authors":["Boucard AA","Arac D","Sudhof TC","Zhang C","Ko J","Brunger AT"],"additional_accession":[]},"is_claimable":false,"name":"Neuroligin-1 performs neurexin-dependent and neurexin-independent functions in synapse validation.","description":"Postsynaptic neuroligins are thought to perform essential functions in synapse validation and synaptic transmission by binding to, and dimerizing, presynaptic alpha- and beta-neurexins. To test this hypothesis, we examined the functional effects of neuroligin-1 mutations that impair only alpha-neurexin binding, block both alpha- and beta-neurexin binding, or abolish neuroligin-1 dimerization. Abolishing alpha-neurexin binding abrogated neuroligin-induced generation of neuronal synapses onto transfected non-neuronal cells in the so-called artificial synapse-formation assay, even though beta-neurexin binding was retained. Thus, in this assay, neuroligin-1 induces apparent synapse formation by binding to presynaptic alpha-neurexins. In transfected neurons, however, neither alpha- nor beta-neu","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Oct","modification":"2026-03-16T16:56:07.248Z","creation":"2025-09-01T03:06:25.586Z"},"accession":"S-EPMC2771087","cross_references":{"pubmed":["19730411"],"doi":["10.1038/emboj.2009.249"]}}