biostudies-literatureUnknown29(22)Zhang JStabilin-1 is a unique scavenger receptor that combines endocytic and intracellular sorting functions in macrophages. Stabilin-1 mediates the endocytosis of acetylated low-density lipoprotein (acLDL), SPARC, and growth hormone family member placental lactogen (PL). At the same time, stabilin-1 is involved in trans-Golgi network-to-endosome routing of the endogenous chitinase-like protein SI-CLP (stabilin-interacting chitinase-like protein). A DDSLL motif in the cytoplasmic tail of stabilin-1 interacts with GGA adaptors; however, the deletion of DDSLL reduces but does not abrogate this interaction. Here, we identified a novel GGA-binding site, EDDADDD, in the cytoplasmic tail of stabilin-1. The deletion of EDDADDD impaired and the deletion of both the DDSLL and EDDADDD sites abrogated the iMolecular and cellular biology6097-105https://www.ebi.ac.uk/biostudies/studies/S-EPMC2772567biostudies-literatureA novel GGA-binding site is required for intracellular sorting mediated by stabilin-1.PMC2772567Gratchev ASage EHSchmuttermaier CZhang JMamidi SKrusell LKremmer EKzhyshkowska JRiabov VJalkanen SWorkman GGoerdt SfalseA novel GGA-binding site is required for intracellular sorting mediated by stabilin-1.Stabilin-1 is a unique scavenger receptor that combines endocytic and intracellular sorting functions in macrophages. Stabilin-1 mediates the endocytosis of acetylated low-density lipoprotein (acLDL), SPARC, and growth hormone family member placental lactogen (PL). At the same time, stabilin-1 is involved in trans-Golgi network-to-endosome routing of the endogenous chitinase-like protein SI-CLP (stabilin-interacting chitinase-like protein). A DDSLL motif in the cytoplasmic tail of stabilin-1 interacts with GGA adaptors; however, the deletion of DDSLL reduces but does not abrogate this interaction. Here, we identified a novel GGA-binding site, EDDADDD, in the cytoplasmic tail of stabilin-1. The deletion of EDDADDD impaired and the deletion of both the DDSLL and EDDADDD sites abrogated the i2009-01-01T00:00:00Z2009 Nov2025-04-18T15:07:44.289Z2019-03-27T00:26:21ZS-EPMC27725671975219710.1128/mcb.00505-0910.1128/MCB.00505-09