biostudies-literatureUnknown10Queiroz R<h4>Background</h4>Trypanosome gene expression is regulated almost exclusively at the post-transcriptional level, with mRNA degradation playing a decisive role. When trypanosomes are transferred from the blood of a mammal to the midgut of a Tsetse fly, they transform to procyclic forms: gene expression is reprogrammed, changing the cell surface and switching the mode of energy metabolism. Within the blood, trypanosomes can pre-adapt for Tsetse transmission, becoming growth-arrested stumpy forms. We describe here the transitions in gene expression that occur during differentiation of in-vitro cultured bloodstream forms to procyclic forms.<h4>Results</h4>Some mRNAs showed changes within 30 min of cis-aconitate addition, whereas others responded 12-24 hours later. For the first 12 h after addBMC genomics495https://www.ebi.ac.uk/biostudies/studies/S-EPMC2772864biostudies-literatureTranscriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons.PMC2772864Benz CClayton CFellenberg KQueiroz RHoheisel JDfalseTranscriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons.<h4>Background</h4>Trypanosome gene expression is regulated almost exclusively at the post-transcriptional level, with mRNA degradation playing a decisive role. When trypanosomes are transferred from the blood of a mammal to the midgut of a Tsetse fly, they transform to procyclic forms: gene expression is reprogrammed, changing the cell surface and switching the mode of energy metabolism. Within the blood, trypanosomes can pre-adapt for Tsetse transmission, becoming growth-arrested stumpy forms. We describe here the transitions in gene expression that occur during differentiation of in-vitro cultured bloodstream forms to procyclic forms.<h4>Results</h4>Some mRNAs showed changes within 30 min of cis-aconitate addition, whereas others responded 12-24 hours later. For the first 12 h after add2009-01-01T00:00:00Z2009 Oct2026-04-18T13:36:05.478Z2026-04-07T14:41:14.771ZS-EPMC27728641985726310.1186/1471-2164-10-495