{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Murani E"],"pubmed_abstract":["<h4>Background</h4>Secreted phosphoprotein 1 (SPP1 or Osteopontin, OPN) is a multifunctional matricellular glycoprotein involved in development and regeneration of skeletal muscle. Previously, we have demonstrated that porcine SPP1 shows breed-related differential mRNA expression during myogenesis. With the aim to identify putative contributing cis-regulatory DNA variation we resequenced the 5' upstream region of the gene in the respective breeds Pietrain and Duroc. We found two single nucleotide polymorphisms (SNP; [GenBank:M84121]: g.1804C>T and g.3836A>G). We focused our investigation on the SNP g.3836A>G, because in silico analysis and knowledge about the regulation of SPP1 suggested an effect of this SNP on a CCAAT/enhancer binding protein beta (C/EBPbeta) responsive transcriptional e"],"journal":["BMC molecular biology"],"pagination":["96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2773772"],"repository":["biostudies-literature"],"pubmed_title":["Dual effect of a single nucleotide polymorphism in the first intron of the porcine secreted phosphoprotein 1 gene: allele-specific binding of C/EBP beta and activation of aberrant splicing."],"pmcid":["PMC2773772"],"pubmed_authors":["Murani E","Seyfert HM","Ponsuksili S","Wimmers K","Shi X"],"additional_accession":[]},"is_claimable":false,"name":"Dual effect of a single nucleotide polymorphism in the first intron of the porcine secreted phosphoprotein 1 gene: allele-specific binding of C/EBP beta and activation of aberrant splicing.","description":"<h4>Background</h4>Secreted phosphoprotein 1 (SPP1 or Osteopontin, OPN) is a multifunctional matricellular glycoprotein involved in development and regeneration of skeletal muscle. Previously, we have demonstrated that porcine SPP1 shows breed-related differential mRNA expression during myogenesis. With the aim to identify putative contributing cis-regulatory DNA variation we resequenced the 5' upstream region of the gene in the respective breeds Pietrain and Duroc. We found two single nucleotide polymorphisms (SNP; [GenBank:M84121]: g.1804C>T and g.3836A>G). We focused our investigation on the SNP g.3836A>G, because in silico analysis and knowledge about the regulation of SPP1 suggested an effect of this SNP on a CCAAT/enhancer binding protein beta (C/EBPbeta) responsive transcriptional e","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Oct","modification":"2026-04-07T15:27:34.572Z","creation":"2019-03-27T00:26:24Z"},"accession":"S-EPMC2773772","cross_references":{"pubmed":["19845957"],"doi":["10.1186/1471-2199-10-96"]}}