biostudies-literatureWittmann DMEuropean Commission FP7e1000569https://www.ebi.ac.uk/biostudies/studies/S-EPMC2774268biostudies-literatureUnknown5(11)The isthmic organizer mediating differentiation of mid- and hindbrain during vertebrate development is characterized by a well-defined pattern of locally restricted gene expression domains around the mid-hindbrain boundary (MHB). This pattern is established and maintained by a regulatory network between several transcription and secreted factors that is not yet understood in full detail. In this contribution we show that a Boolean analysis of the characteristic spatial gene expression patterns at the murine MHB reveals key regulatory interactions in this network. Our analysis employs techniques from computational logic for the minimization of Boolean functions. This approach allows us to predict also the interplay of the various regulatory interactions. In particular, we predict a maintainPLoS computational biologySpatial analysis of expression patterns predicts genetic interactions at the mid-hindbrain boundary.PMC2774268FP7_222999Prakash NWittmann DMBlochl FTheis FJTrumbach DWurst WfalseSpatial analysis of expression patterns predicts genetic interactions at the mid-hindbrain boundary.The isthmic organizer mediating differentiation of mid- and hindbrain during vertebrate development is characterized by a well-defined pattern of locally restricted gene expression domains around the mid-hindbrain boundary (MHB). This pattern is established and maintained by a regulatory network between several transcription and secreted factors that is not yet understood in full detail. In this contribution we show that a Boolean analysis of the characteristic spatial gene expression patterns at the murine MHB reveals key regulatory interactions in this network. Our analysis employs techniques from computational logic for the minimization of Boolean functions. This approach allows us to predict also the interplay of the various regulatory interactions. In particular, we predict a maintain2009-01-01T00:00:00Z2009 Nov2026-07-15T07:04:29.449Z2025-09-01T03:06:37.399ZS-EPMC27742681993605910.1371/journal.pcbi.1000569