{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Loftus LT"],"funding":["NINDS NIH HHS"],"pagination":["71-80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2774733"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1272"],"pubmed_abstract":["Several recent studies suggest that sumo-2/3 modification of proteins occurs following harmful ischemia, however, sumo-2/3-ylation may also be associated with hibernation-mediated neuroprotection. Here we investigate the sumoylation of proteins following ischemia and ischemic tolerance using our established in vitro model of ischemia (oxygen and glucose deprivation; OGD). Following harmful ischemia (120 min OGD), we observed a significant increase in the sumo-2/3-ylation of high molecular weight proteins (>85 kDa), but not sumo-1-ylation of proteins. Sumo-2/3-ylation following 120 min OGD was reduced when cultures were preconditioned with non-harmful 30 min OGD 24 h earlier (delayed ischemic tolerance). However, we observed no change in sumo-2/3-ylation in a model of rapid ischemic toleran"],"journal":["Brain research"],"pubmed_title":["Sumo-2/3-ylation following in vitro modeled ischemia is reduced in delayed ischemic tolerance."],"pmcid":["PMC2774733"],"funding_grant_id":["R21 NS054023-02","R01 NS059588-01A2","NS024728","R01 NS059588","NS059588","R01 NS024728","NS054023","R21 NS050669","R21 NS054023","R01 NS024728-22"],"pubmed_authors":["Gala R","Ordonez AN","Jessick VJ","Thompson SJ","Simon RP","Meller R","Yang T","Ashley MD","Loftus LT"],"additional_accession":[]},"is_claimable":false,"name":"Sumo-2/3-ylation following in vitro modeled ischemia is reduced in delayed ischemic tolerance.","description":"Several recent studies suggest that sumo-2/3 modification of proteins occurs following harmful ischemia, however, sumo-2/3-ylation may also be associated with hibernation-mediated neuroprotection. Here we investigate the sumoylation of proteins following ischemia and ischemic tolerance using our established in vitro model of ischemia (oxygen and glucose deprivation; OGD). Following harmful ischemia (120 min OGD), we observed a significant increase in the sumo-2/3-ylation of high molecular weight proteins (>85 kDa), but not sumo-1-ylation of proteins. Sumo-2/3-ylation following 120 min OGD was reduced when cultures were preconditioned with non-harmful 30 min OGD 24 h earlier (delayed ischemic tolerance). However, we observed no change in sumo-2/3-ylation in a model of rapid ischemic toleran","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 May","modification":"2025-04-04T13:38:27.544Z","creation":"2019-03-27T00:26:26Z"},"accession":"S-EPMC2774733","cross_references":{"pubmed":["19332039"],"doi":["10.1016/j.brainres.2009.03.034"]}}