biostudies-literatureLoftus LTNINDS NIH HHS71-80https://www.ebi.ac.uk/biostudies/studies/S-EPMC2774733biostudies-literatureUnknown1272Several recent studies suggest that sumo-2/3 modification of proteins occurs following harmful ischemia, however, sumo-2/3-ylation may also be associated with hibernation-mediated neuroprotection. Here we investigate the sumoylation of proteins following ischemia and ischemic tolerance using our established in vitro model of ischemia (oxygen and glucose deprivation; OGD). Following harmful ischemia (120 min OGD), we observed a significant increase in the sumo-2/3-ylation of high molecular weight proteins (>85 kDa), but not sumo-1-ylation of proteins. Sumo-2/3-ylation following 120 min OGD was reduced when cultures were preconditioned with non-harmful 30 min OGD 24 h earlier (delayed ischemic tolerance). However, we observed no change in sumo-2/3-ylation in a model of rapid ischemic toleranBrain researchSumo-2/3-ylation following in vitro modeled ischemia is reduced in delayed ischemic tolerance.PMC2774733R21 NS054023-02R01 NS059588-01A2NS024728R01 NS059588NS059588R01 NS024728NS054023R21 NS050669R21 NS054023R01 NS024728-22Gala ROrdonez ANJessick VJThompson SJSimon RPMeller RYang TAshley MDLoftus LTfalseSumo-2/3-ylation following in vitro modeled ischemia is reduced in delayed ischemic tolerance.Several recent studies suggest that sumo-2/3 modification of proteins occurs following harmful ischemia, however, sumo-2/3-ylation may also be associated with hibernation-mediated neuroprotection. Here we investigate the sumoylation of proteins following ischemia and ischemic tolerance using our established in vitro model of ischemia (oxygen and glucose deprivation; OGD). Following harmful ischemia (120 min OGD), we observed a significant increase in the sumo-2/3-ylation of high molecular weight proteins (>85 kDa), but not sumo-1-ylation of proteins. Sumo-2/3-ylation following 120 min OGD was reduced when cultures were preconditioned with non-harmful 30 min OGD 24 h earlier (delayed ischemic tolerance). However, we observed no change in sumo-2/3-ylation in a model of rapid ischemic toleran2009-01-01T00:00:00Z2009 May2025-04-04T13:38:27.544Z2019-03-27T00:26:26ZS-EPMC27747331933203910.1016/j.brainres.2009.03.034