{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28"],"submitter":["Liu Z"],"pubmed_abstract":["<h4>Background</h4>CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2).<h4>Methods</h4>We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation in"],"journal":["Journal of experimental & clinical cancer research : CR"],"pagination":["156"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2804602"],"repository":["biostudies-literature"],"pubmed_title":["Reduced expression of cenp-e in human hepatocellular carcinoma."],"pmcid":["PMC2804602"],"pubmed_authors":["Si Q","Liu Z","Li S","Cao J","Zhang Y","Weng Y","Cai Y","Ling K","Liu B","Yan C","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Reduced expression of cenp-e in human hepatocellular carcinoma.","description":"<h4>Background</h4>CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2).<h4>Methods</h4>We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation in","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Dec","modification":"2025-04-25T23:15:49.534Z","creation":"2019-06-06T21:49:59Z"},"accession":"S-EPMC2804602","cross_references":{"pubmed":["20021663"],"doi":["10.1186/1756-9966-28-156"]}}