biostudies-literatureUnknown28Liu Z<h4>Background</h4>CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2).<h4>Methods</h4>We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation inJournal of experimental & clinical cancer research : CR156https://www.ebi.ac.uk/biostudies/studies/S-EPMC2804602biostudies-literatureReduced expression of cenp-e in human hepatocellular carcinoma.PMC2804602Si QLiu ZLi SCao JZhang YWeng YCai YLing KLiu BYan CWu XfalseReduced expression of cenp-e in human hepatocellular carcinoma.<h4>Background</h4>CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2).<h4>Methods</h4>We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation in2009-01-01T00:00:00Z2009 Dec2025-04-25T23:15:49.534Z2019-06-06T21:49:59ZS-EPMC28046022002166310.1186/1756-9966-28-156