{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chatterjee A"],"funding":["NIDDK NIH HHS","NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["84-96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2805294"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(1)"],"pubmed_abstract":["Cryptosporidium parvum oocysts, which are spread by the fecal-oral route, have a single, multilayered wall that surrounds four sporozoites, the invasive form. The C. parvum oocyst wall is labeled by the Maclura pomifera agglutinin (MPA), which binds GalNAc, and the C. parvum wall contains at least two unique proteins (Cryptosporidium oocyst wall protein 1 [COWP1] and COWP8) identified by monoclonal antibodies. C. parvum sporozoites have on their surface multiple mucin-like glycoproteins with Ser- and Thr-rich repeats (e.g., gp40 and gp900). Here we used ruthenium red staining and electron microscopy to demonstrate fibrils, which appear to attach or tether sporozoites to the inner surface of the C. parvum oocyst wall. When disconnected from the sporozoites, some of these fibrillar tethers a"],"journal":["Eukaryotic cell"],"pubmed_title":["Evidence for mucin-like glycoproteins that tether sporozoites of Cryptosporidium parvum to the inner surface of the oocyst wall."],"pmcid":["PMC2805294"],"funding_grant_id":["GM31318","R01 AI044070","R01 GM031318","AI52786","DK62816","K01 DK062816","AI44070","R01 AI052786"],"pubmed_authors":["Banerjee S","Samuelson J","Robbins PW","Steffen M","O'Connor RM","Ward HD","Chatterjee A"],"additional_accession":[]},"is_claimable":false,"name":"Evidence for mucin-like glycoproteins that tether sporozoites of Cryptosporidium parvum to the inner surface of the oocyst wall.","description":"Cryptosporidium parvum oocysts, which are spread by the fecal-oral route, have a single, multilayered wall that surrounds four sporozoites, the invasive form. The C. parvum oocyst wall is labeled by the Maclura pomifera agglutinin (MPA), which binds GalNAc, and the C. parvum wall contains at least two unique proteins (Cryptosporidium oocyst wall protein 1 [COWP1] and COWP8) identified by monoclonal antibodies. C. parvum sporozoites have on their surface multiple mucin-like glycoproteins with Ser- and Thr-rich repeats (e.g., gp40 and gp900). Here we used ruthenium red staining and electron microscopy to demonstrate fibrils, which appear to attach or tether sporozoites to the inner surface of the C. parvum oocyst wall. When disconnected from the sporozoites, some of these fibrillar tethers a","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jan","modification":"2025-04-22T11:31:56.516Z","creation":"2019-03-27T00:27:53Z"},"accession":"S-EPMC2805294","cross_references":{"pubmed":["19949049"],"doi":["10.1128/EC.00288-09","10.1128/ec.00288-09"]}}