{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["192(3)"],"submitter":["Bekker M"],"pubmed_abstract":["ArcBA is a two-component regulatory system of Escherichia coli involved in sensing oxygen availability and the concomitant transcriptional regulation of oxidative and fermentative catabolism. Based on in vitro data, it has been postulated that the redox state of the ubiquinone pool is the determinant for ArcB kinase activity. Here we report on the in vivo regulation of ArcB activation, as determined using a lacZ reporter specifically responsive to phosphorylated ArcA. Our results indicate that upon deletion of a ubiquinone biosynthetic enzyme, regulation of ArcB in the anaerobic-aerobic transition is not affected. In contrast, interference with menaquinone biosynthesis leads to inactivation of ArcB during anaerobic growth; this phenotype is fully rescued by addition of a menaquinone precur"],"journal":["Journal of bacteriology"],"pagination":["746-54"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2812447"],"repository":["biostudies-literature"],"pubmed_title":["The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool."],"pmcid":["PMC2812447"],"pubmed_authors":["Bekker M","Hellingwerf K","Sawers G","Alexeeva S","Teixeira de Mattos J","Laan W"],"additional_accession":[]},"is_claimable":false,"name":"The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool.","description":"ArcBA is a two-component regulatory system of Escherichia coli involved in sensing oxygen availability and the concomitant transcriptional regulation of oxidative and fermentative catabolism. Based on in vitro data, it has been postulated that the redox state of the ubiquinone pool is the determinant for ArcB kinase activity. Here we report on the in vivo regulation of ArcB activation, as determined using a lacZ reporter specifically responsive to phosphorylated ArcA. Our results indicate that upon deletion of a ubiquinone biosynthetic enzyme, regulation of ArcB in the anaerobic-aerobic transition is not affected. In contrast, interference with menaquinone biosynthesis leads to inactivation of ArcB during anaerobic growth; this phenotype is fully rescued by addition of a menaquinone precur","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Feb","modification":"2026-04-29T13:13:30.331Z","creation":"2025-05-29T22:30:39.439Z"},"accession":"S-EPMC2812447","cross_references":{"pubmed":["19933363"],"doi":["10.1128/JB.01156-09","10.1128/jb.01156-09"]}}