{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["192(5)"],"submitter":["Keese AM"],"pubmed_abstract":["The hyperthermophilic archaeon Pyrococcus furiosus grows optimally near 100 degrees C and undergoes a heat shock response at 105 degrees C, mediated at least in part by the heat shock regulator Phr. Genes encoding a small heat shock protein (HSP20) and a member of the AAA(+) ATPase are the only known targets of the regulator, but a genetic mutant of Phr has yet to be characterized. We describe here an alternative approach for the identification of the regulon of Phr based on cell-free transcription of fragmented chromosomal DNA in the presence or absence of the regulator and hybridization of in vitro RNA to P. furiosus whole-genome microarrays. Our results confirmed the phr, the hsp20, and the aaa(+) ATPase genes as targets of Phr and also identified six additional open reading frames, PF0"],"journal":["Journal of bacteriology"],"pagination":["1292-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2820856"],"repository":["biostudies-literature"],"pubmed_title":["Genome-wide identification of targets for the archaeal heat shock regulator phr by cell-free transcription of genomic DNA."],"pmcid":["PMC2820856"],"pubmed_authors":["Adams MW","Schut GJ","Keese AM","Thomm M","Ouhammouch M"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide identification of targets for the archaeal heat shock regulator phr by cell-free transcription of genomic DNA.","description":"The hyperthermophilic archaeon Pyrococcus furiosus grows optimally near 100 degrees C and undergoes a heat shock response at 105 degrees C, mediated at least in part by the heat shock regulator Phr. Genes encoding a small heat shock protein (HSP20) and a member of the AAA(+) ATPase are the only known targets of the regulator, but a genetic mutant of Phr has yet to be characterized. We describe here an alternative approach for the identification of the regulon of Phr based on cell-free transcription of fragmented chromosomal DNA in the presence or absence of the regulator and hybridization of in vitro RNA to P. furiosus whole-genome microarrays. Our results confirmed the phr, the hsp20, and the aaa(+) ATPase genes as targets of Phr and also identified six additional open reading frames, PF0","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Mar","modification":"2025-04-26T04:19:48.057Z","creation":"2019-03-27T00:28:33Z"},"accession":"S-EPMC2820856","cross_references":{"pubmed":["20023014"],"doi":["10.1128/JB.00924-09","10.1128/jb.00924-09"]}}