biostudies-literatureUnknown192(5)Keese AMThe hyperthermophilic archaeon Pyrococcus furiosus grows optimally near 100 degrees C and undergoes a heat shock response at 105 degrees C, mediated at least in part by the heat shock regulator Phr. Genes encoding a small heat shock protein (HSP20) and a member of the AAA(+) ATPase are the only known targets of the regulator, but a genetic mutant of Phr has yet to be characterized. We describe here an alternative approach for the identification of the regulon of Phr based on cell-free transcription of fragmented chromosomal DNA in the presence or absence of the regulator and hybridization of in vitro RNA to P. furiosus whole-genome microarrays. Our results confirmed the phr, the hsp20, and the aaa(+) ATPase genes as targets of Phr and also identified six additional open reading frames, PF0Journal of bacteriology1292-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC2820856biostudies-literatureGenome-wide identification of targets for the archaeal heat shock regulator phr by cell-free transcription of genomic DNA.PMC2820856Adams MWSchut GJKeese AMThomm MOuhammouch MfalseGenome-wide identification of targets for the archaeal heat shock regulator phr by cell-free transcription of genomic DNA.The hyperthermophilic archaeon Pyrococcus furiosus grows optimally near 100 degrees C and undergoes a heat shock response at 105 degrees C, mediated at least in part by the heat shock regulator Phr. Genes encoding a small heat shock protein (HSP20) and a member of the AAA(+) ATPase are the only known targets of the regulator, but a genetic mutant of Phr has yet to be characterized. We describe here an alternative approach for the identification of the regulon of Phr based on cell-free transcription of fragmented chromosomal DNA in the presence or absence of the regulator and hybridization of in vitro RNA to P. furiosus whole-genome microarrays. Our results confirmed the phr, the hsp20, and the aaa(+) ATPase genes as targets of Phr and also identified six additional open reading frames, PF02010-01-01T00:00:00Z2010 Mar2025-04-26T04:19:48.057Z2019-03-27T00:28:33ZS-EPMC28208562002301410.1128/JB.00924-0910.1128/jb.00924-09