biostudies-literatureZhang NBiotechnology and Biological Sciences Research Council6465-76https://www.ebi.ac.uk/biostudies/studies/S-EPMC2825442biostudies-literatureUnknown285(9)The transcriptional response to environmental changes has to be prompt but appropriate. Previously, it has been shown that the Gis1 transcription factor is responsible for regulating the expression of postdiauxic shift genes in response to nutrient starvation, and this transcription regulation is dependent upon the Rim15 kinase. Here we demonstrate that the activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. Limited degradation of Gis1 by the proteasome leads to the production of smaller variants, which have weaker transcription activities than the full-length protein. The coiled-coil domain, absent from the smaller variants, is part of the second transcription activation domain in Gis1 and is essential for both the limited proteolysis of Gis1 and its full The Journal of biological chemistryThe transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis.PMC2825442BB/C505140/1BB/C505140/2Oliver SGZhang NfalseThe transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis.The transcriptional response to environmental changes has to be prompt but appropriate. Previously, it has been shown that the Gis1 transcription factor is responsible for regulating the expression of postdiauxic shift genes in response to nutrient starvation, and this transcription regulation is dependent upon the Rim15 kinase. Here we demonstrate that the activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. Limited degradation of Gis1 by the proteasome leads to the production of smaller variants, which have weaker transcription activities than the full-length protein. The coiled-coil domain, absent from the smaller variants, is part of the second transcription activation domain in Gis1 and is essential for both the limited proteolysis of Gis1 and its full 2010-01-01T00:00:00Z2010 Feb2026-04-14T21:26:23.255Z2019-03-27T00:28:47ZS-EPMC28254422002295310.1074/jbc.M109.07328810.1074/jbc.m109.073288