{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["58(3)"],"submitter":["Paavilainen L"],"pubmed_abstract":["Pathology archives harbor large amounts of formalin-fixed, paraffin-embedded tissue samples, used mainly in clinical diagnostics but also for research purposes. Introduction of heat-induced antigen retrieval has enabled the use of tissue samples for extensive immunohistochemical analysis, despite the fact that antigen retrieval may not recover all epitopes, owing to alterations of the native protein structure induced by formalin. The aim of this study was to investigate how different fixatives influence protein recognition by immunodetection methods in tissues, cell preparations, and protein lysates, as compared with formalin. Seventy-two affinity-purified polyclonal antibodies were used to evaluate seven different fixatives. The aldehyde-based fixative Glyo-fixx proved to be excellent for"],"journal":["The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society"],"pagination":["237-46"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2825489"],"repository":["biostudies-literature"],"pubmed_title":["The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells."],"pmcid":["PMC2825489"],"pubmed_authors":["Edvinsson A","Hober S","Paavilainen L","Ponten F","Wester K","Kampf C","Asplund A"],"additional_accession":[]},"is_claimable":false,"name":"The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells.","description":"Pathology archives harbor large amounts of formalin-fixed, paraffin-embedded tissue samples, used mainly in clinical diagnostics but also for research purposes. Introduction of heat-induced antigen retrieval has enabled the use of tissue samples for extensive immunohistochemical analysis, despite the fact that antigen retrieval may not recover all epitopes, owing to alterations of the native protein structure induced by formalin. The aim of this study was to investigate how different fixatives influence protein recognition by immunodetection methods in tissues, cell preparations, and protein lysates, as compared with formalin. Seventy-two affinity-purified polyclonal antibodies were used to evaluate seven different fixatives. The aldehyde-based fixative Glyo-fixx proved to be excellent for","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Mar","modification":"2025-05-31T22:36:56.967Z","creation":"2019-06-06T21:54:06Z"},"accession":"S-EPMC2825489","cross_references":{"pubmed":["19901271"],"doi":["10.1369/jhc.2009.954321"]}}