biostudies-literatureUnknown82(3)Campuzano-Zuluaga GThick film, the standard diagnostic procedure for malaria, is not always ordered promptly. A failsafe diagnostic strategy using an XE-2100 analyzer is proposed, and for this strategy, malaria diagnostic models for the XE-2100 were developed and tested for accuracy. Two hundred eighty-one samples were distributed into Plasmodium vivax, P. falciparum, and acute febrile syndrome groups for model construction. Model validation was performed using 60% of malaria cases and a composite control group of samples from AFS and healthy participants from endemic and non-endemic regions. For P. vivax, two observer-dependent models (accuracy = 95.3-96.9%), one non-observer-dependent model using built-in variables (accuracy = 94.7%), and one non-observer-dependent model using new and built-in variables (accuracy = 96.8%) were developed. For P. falciparum, two non-observer-dependent models (accuracies = 85% and 89%) were developed. These models could be used by health personnel or be integrated as a malaria alarm for the XE-2100 to prompt early malaria microscopic diagnosis.The American journal of tropical medicine and hygiene402-11https://www.ebi.ac.uk/biostudies/studies/S-EPMC2829900biostudies-literatureDesign of malaria diagnostic criteria for the Sysmex XE-2100 hematology analyzer.PMC2829900Alvarez-Sanchez GValencia-Zuluaga LMEscobar-Gallo GEPabon-Vidal ARios-Orrego AMCampuzano-Zuluaga GBlair-Trujillo SCampuzano-Maya GMiranda-Arboleda AFfalseDesign of malaria diagnostic criteria for the Sysmex XE-2100 hematology analyzer.Thick film, the standard diagnostic procedure for malaria, is not always ordered promptly. A failsafe diagnostic strategy using an XE-2100 analyzer is proposed, and for this strategy, malaria diagnostic models for the XE-2100 were developed and tested for accuracy. Two hundred eighty-one samples were distributed into Plasmodium vivax, P. falciparum, and acute febrile syndrome groups for model construction. Model validation was performed using 60% of malaria cases and a composite control group of samples from AFS and healthy participants from endemic and non-endemic regions. For P. vivax, two observer-dependent models (accuracy = 95.3-96.9%), one non-observer-dependent model using built-in variables (accuracy = 94.7%), and one non-observer-dependent model using new and built-in variables (accuracy = 96.8%) were developed. For P. falciparum, two non-observer-dependent models (accuracies = 85% and 89%) were developed. These models could be used by health personnel or be integrated as a malaria alarm for the XE-2100 to prompt early malaria microscopic diagnosis.2010-01-01T00:00:00Z2010 Mar2025-04-18T23:13:44.873Z2019-03-27T00:28:56ZS-EPMC28299002020786410.4269/ajtmh.2010.09-0464