biostudies-literatureAsolkar RNFIC NIH HHSNCI NIH HHS396-402https://www.ebi.ac.uk/biostudies/studies/S-EPMC2837138biostudies-literatureUnknown72(3)Three new cyclohexadepsipeptides, arenamides A-C (1-3), were isolated from the fermentation broth of a marine bacterial strain identified as Salinispora arenicola. The planar structures of these compounds were assigned by detailed interpretation of NMR and MS/MS spectroscopic data. The absolute configurations of the amino acids, and those of the chiral centers on the side chain, were established by application of the Marfey and modified Mosher methods. The effect of arenamides A and B on NFkappaB activity was studied with stably transfected 293/NFkappaB-Luc human embryonic kidney cells induced by treatment with tumor necrosis factor (TNF). Arenamides A (1) and B (2) blocked TNF-induced activation in a dose- and time-dependent manner with IC(50) values of 3.7 and 1.7 microM, respectively. IJournal of natural productsArenamides A-C, cytotoxic NFkappaB inhibitors from the marine actinomycete Salinispora arenicola.PMC2837138P01 CA048112-150007R37 CA044848-22R37 CA044848U01 TW007401P01 CA048112P01 CA48112U01-TW007401-01Kondratyuk TPJensen PRFenical WAsolkar RNFreel KCPark EJPezzuto JMfalseArenamides A-C, cytotoxic NFkappaB inhibitors from the marine actinomycete Salinispora arenicola.Three new cyclohexadepsipeptides, arenamides A-C (1-3), were isolated from the fermentation broth of a marine bacterial strain identified as Salinispora arenicola. The planar structures of these compounds were assigned by detailed interpretation of NMR and MS/MS spectroscopic data. The absolute configurations of the amino acids, and those of the chiral centers on the side chain, were established by application of the Marfey and modified Mosher methods. The effect of arenamides A and B on NFkappaB activity was studied with stably transfected 293/NFkappaB-Luc human embryonic kidney cells induced by treatment with tumor necrosis factor (TNF). Arenamides A (1) and B (2) blocked TNF-induced activation in a dose- and time-dependent manner with IC(50) values of 3.7 and 1.7 microM, respectively. I2009-01-01T00:00:00Z2009 Mar2025-04-04T19:58:41.063Z2019-03-27T00:29:18ZS-EPMC28371381911739910.1021/np800617a