biostudies-literatureYustein JTNCI NIH HHSNINDS NIH HHS3534-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC2840500biostudies-literatureUnknown107(8)Ectopic Myc expression plays a key role in human tumorigenesis, and Myc dose-dependent tumorigenesis has been well established in transgenic mice, but the Myc target genes that are dependent on Myc levels have not been well characterized. In this regard, we used the human P493-6 B cells, which have a preneoplastic state dependent on the Epstein-Barr viral EBNA2 protein and a neoplastic state with ectopic inducible Myc, to identify putative ectopic Myc target genes. Among the ectopic targets, JAG2 that encodes a Notch receptor ligand Jagged2, was directly induced by Myc. Inhibition of Notch signaling through RNAi targeting JAG2 or the gamma-secretase Notch inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester (DAPT) preferentially inhibited the neoplastic state iProceedings of the National Academy of Sciences of the United States of AmericaInduction of ectopic Myc target gene JAG2 augments hypoxic growth and tumorigenesis in a human B-cell model.PMC2840500R01 NS055089R01 CA051497T32CA60441R01 CA57341R01 CA51497T32 CA060441R01 CA057341Yustein JTGao PChng WJDang CVVuica-Ross MJie CLe AEberhart CGLiu YCBergsagel PLfalseInduction of ectopic Myc target gene JAG2 augments hypoxic growth and tumorigenesis in a human B-cell model.Ectopic Myc expression plays a key role in human tumorigenesis, and Myc dose-dependent tumorigenesis has been well established in transgenic mice, but the Myc target genes that are dependent on Myc levels have not been well characterized. In this regard, we used the human P493-6 B cells, which have a preneoplastic state dependent on the Epstein-Barr viral EBNA2 protein and a neoplastic state with ectopic inducible Myc, to identify putative ectopic Myc target genes. Among the ectopic targets, JAG2 that encodes a Notch receptor ligand Jagged2, was directly induced by Myc. Inhibition of Notch signaling through RNAi targeting JAG2 or the gamma-secretase Notch inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester (DAPT) preferentially inhibited the neoplastic state i2010-01-01T00:00:00Z2010 Feb2026-05-01T16:58:14.11Z2026-04-18T03:08:35.571ZS-EPMC28405002013358510.1073/pnas.0901230107