biostudies-literatureGnjatic SNCI NIH HHS5088-93https://www.ebi.ac.uk/biostudies/studies/S-EPMC2841879biostudies-literatureUnknown107(11)Autoantibodies, a hallmark of both autoimmunity and cancer, represent an easily accessible surrogate for measuring adaptive immune responses to cancer. Sera can now be assayed for reactivity against thousands of proteins using microarrays, but there is no agreed-upon standard to analyze results. We developed a set of tailored quality control and normalization procedures based on ELISA validation to allow patient comparisons and determination of individual cutoffs for specificity and sensitivity. Sera from 60 patients with pancreatic cancer, 51 patients with ovarian cancer, and 53 age-matched healthy donors were used to assess the binding of IgG antibodies against a panel of >8000 human antigens using protein microarrays and fluorescence detection. The resulting data interpretation led to tProceedings of the National Academy of Sciences of the United States of AmericaSeromic profiling of ovarian and pancreatic cancer.PMC2841879P30 CA016056CA16056Giese NABuchler MWGnjatic SAndrews CJager DMurray AHalama NChen YTBrors BRitter GOld LJOdunsi KRitter EFrei CZornig IfalseSeromic profiling of ovarian and pancreatic cancer.Autoantibodies, a hallmark of both autoimmunity and cancer, represent an easily accessible surrogate for measuring adaptive immune responses to cancer. Sera can now be assayed for reactivity against thousands of proteins using microarrays, but there is no agreed-upon standard to analyze results. We developed a set of tailored quality control and normalization procedures based on ELISA validation to allow patient comparisons and determination of individual cutoffs for specificity and sensitivity. Sera from 60 patients with pancreatic cancer, 51 patients with ovarian cancer, and 53 age-matched healthy donors were used to assess the binding of IgG antibodies against a panel of >8000 human antigens using protein microarrays and fluorescence detection. The resulting data interpretation led to t2010-01-01T00:00:00Z2010 Mar2026-07-15T14:27:54.417Z2019-03-27T00:29:32ZS-EPMC28418792019476510.1073/pnas.0914213107