{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["285(13)"],"submitter":["Courtois ET"],"pubmed_abstract":["Human neural stem cells derived from the ventral mesencephalon (VM) are powerful research tools and candidates for cell therapies in Parkinson disease. Previous studies with VM dopaminergic neuron (DAn) precursors indicated poor growth potential and unstable phenotypical properties. Using the model cell line hVM1 (human ventral mesencephalic neural stem cell line 1; a new human fetal VM stem cell line), we have found that Bcl-X(L) enhances the generation of DAn from VM human neural stem cells. Mechanistically, Bcl-X(L) not only exerts the expected antiapoptotic effect but also induces proneural (NGN2 and NEUROD1) and dopamine-related transcription factors, resulting in a high yield of DAn with the correct phenotype of substantia nigra pars compacta (SNpc). The expression of key genes direc"],"journal":["The Journal of biological chemistry"],"pagination":["9881-9897"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2843236"],"repository":["biostudies-literature"],"pubmed_title":["In vitro and in vivo enhanced generation of human A9 dopamine neurons from neural stem cells by Bcl-XL."],"pmcid":["PMC2843236"],"pubmed_authors":["Seiz EG","Liste I","Bueno C","Ramos M","Courtois ET","Martinez-Serrano A","Castillo CG"],"additional_accession":[]},"is_claimable":false,"name":"In vitro and in vivo enhanced generation of human A9 dopamine neurons from neural stem cells by Bcl-XL.","description":"Human neural stem cells derived from the ventral mesencephalon (VM) are powerful research tools and candidates for cell therapies in Parkinson disease. Previous studies with VM dopaminergic neuron (DAn) precursors indicated poor growth potential and unstable phenotypical properties. Using the model cell line hVM1 (human ventral mesencephalic neural stem cell line 1; a new human fetal VM stem cell line), we have found that Bcl-X(L) enhances the generation of DAn from VM human neural stem cells. Mechanistically, Bcl-X(L) not only exerts the expected antiapoptotic effect but also induces proneural (NGN2 and NEUROD1) and dopamine-related transcription factors, resulting in a high yield of DAn with the correct phenotype of substantia nigra pars compacta (SNpc). The expression of key genes direc","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Mar","modification":"2025-05-31T23:14:58.471Z","creation":"2025-05-31T23:14:58.471Z"},"accession":"S-EPMC2843236","cross_references":{"pubmed":["20106970"],"doi":["10.1074/jbc.M109.054312","10.1074/jbc.m109.054312"]}}